PMID: 8358534Jun 1, 1993Paper

High-affinity uptake of noradrenaline in postsynaptic neurones

British Journal of Pharmacology
Saad Al-DamlujiK J Catt

Abstract

1. Neurotransmitters released from nerve endings are inactivated by re-uptake into the presynaptic nerve terminals and possibly into neighbouring glial cells. While analysing the functional properties of alpha 1-adrenoceptors in the hypothalamus, we observed a high-affinity uptake process for noradrenaline in postsynaptic peptidergic neurones. 2. In primary hypothalamic cell cultures and in a hypothalamic neuronal cell line, [3H]-prazosin bound with high affinity and was displaced by unlabelled prazosin in concentrations of 10(-10) to 10(-7) M. However, at concentrations of unlabelled prazosin above 10(-7) M, there was a paradoxical increase in apparent [3H]-prazosin binding. 3. Methoxamine, an alpha 1-adrenoceptor ligand that is not subject to significant neuronal uptake, displaced [3H]-prazosin but did not cause the paradoxical increase in the apparent binding of [3H]-prazosin. Cooling the cells to 4 degrees C reduced the total amount of prazosin associated with the cells; under these conditions, methoxamine almost completely inhibited [3H]-prazosin binding to the cells. 4. In the presence of desipramine (DMI), unlabelled prazosin displaced [3H]-prazosin as before, but no paradoxical increase in apparent binding was seen abov...Continue Reading

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Citations

Oct 18, 2002·Life Sciences·Ana Paula TannoFernanda Klein Marcondes
Mar 1, 1997·British Journal of Pharmacology·S al-DamlujiI J Kopin
Aug 5, 1998·British Journal of Pharmacology·S Al-Damluji, I J Kopin
Jan 13, 2001·British Journal of Pharmacology·S Al-DamlujiE A Barnard
Feb 22, 2001·British Journal of Pharmacology·S Al-Damluji, W B Shen
Jan 1, 1996·British Journal of Pharmacology·S Al-Damluji, I J Kopin
Jul 16, 2008·Bioorganic & Medicinal Chemistry·Joaquim Fernando Mendes da SilvaC Robin Ganellin
May 3, 2005·Bioorganic & Medicinal Chemistry·Patricia A ZunszainC Robin Ganellin
Jul 1, 1994·Trends in Endocrinology and Metabolism : TEM·S S StojilkovicK J Catt

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