High amylose wheat starch structures display unique fermentability characteristics, microbial community shifts and enzyme degradation profiles.

Food & Function
Alexander T BuiMichael J Gidley

Abstract

A slower rate of starch digestion in the small intestine increases the amount of resistant starch (RS) entering the large intestine, which is associated with health benefits. Although increasing the amylose (AM) content of dietary starch intake is one way to increase RS, the processes involved in gut microbial hydrolysis and fermentation of high AM-RS substrates are poorly understood. In this study, five high AM wheat (HAW) starches ranging from 47% AM to 93% AM and a wild type (37% AM), in both native granular and cooked forms, were subjected to in vitro fermentation with a porcine faecal inoculum. Fermentation kinetics, temporal microbial changes, amylolytic enzyme activities and residual starch were determined. All granular starches showed similar fermentation characteristics, independent of AM level, whereas cooking accelerated fermentation of lower AM but slowed fermentation of high AM starches. HAW starches with a very high AM content (>85%) all had similar fermentation kinetics and short-chain fatty acid end-product profiles. Microbial α-amylase, β-amylase, pullulanase and amyloglucosidase enzymatic activities were all detected and followed fermentation kinetics. HAW starch promoted shifts in the microbial community, wit...Continue Reading

References

Apr 16, 2003·The Plant Journal : for Cell and Molecular Biology·Matthew K MorellZhongyi Li
May 22, 2004·BioTechniques·Zhongtang Yu, Mark Morrison
Jul 15, 2005·International Journal of Systematic and Evolutionary Microbiology·Marcel NordhoffLothar H Wieler
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ahmed ReginaMatthew Morell
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Apr 22, 2011·Nature·Manimozhiyan ArumugamPeer Bork
May 26, 2011·Journal of Agricultural and Food Chemistry·Morgan J TizzottiRobert G Gilbert
Nov 29, 2011·Molecular Nutrition & Food Research·Karen WindeyKristin Verbeke
May 11, 2012·Gut Microbes·Harry J FlintEvelyne Forano
May 16, 2012·BMC Plant Biology·Ann J SladeVic C Knauf
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jun 12, 2013·Nature Reviews. Microbiology·Abdessamad El KaoutariBernard Henrissat
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Federico E ReyJeffrey I Gordon
Oct 24, 2013·Current Gastroenterology Reports·Mark PimentelChristopher Chang
Apr 17, 2014·Nature Communications·Stephanie L SchnorrAlyssa N Crittenden
Sep 10, 2014·Nature Reviews. Microbiology·Petra LouisHarry J Flint
Mar 10, 2015·Critical Reviews in Food Science and Nutrition·Sushil DhitalMichael J Gidley
May 27, 2015·Alimentary Pharmacology & Therapeutics·H L Simpson, B J Campbell
Mar 1, 2016·Frontiers in Microbiology·David Ríos-CoviánNuria Salazar
Jul 10, 2016·Journal of Molecular Biology·Darrell W Cockburn, Nicole M Koropatkin
Sep 10, 2016·Food and Energy Security·Peter R Shewry, Sandra J Hey
Jun 3, 2017·Scientific Reports·Tingting ChenBruce R Hamaker
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 18, 2018·MSphere·Frederick J WarrenMichael J Gidley
Jun 15, 2018·Cell Host & Microbe·Kassem MakkiFredrik Bäckhed

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Methods Mentioned

BETA
PCR

Software Mentioned

Calypso
BLASTn
Excel
Quantitative Insight Into Microbial Ecology ( QIIME )
USEARCH
SAS
Linux
MEGA

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