High and low oxygen affinity conformations of T state hemoglobin

Protein Science : a Publication of the Protein Society
S BrunoA Mozzarelli

Abstract

To understand the interplay between tertiary and quaternary transitions associated with hemoglobin function and regulation, oxygen binding curves were obtained for hemoglobin A fixed in the T quaternary state by encapsulation in wet porous silica gels. At pH 7.0 and 15 degrees C, the oxygen pressure at half saturation (p50) was measured to be 12.4 +/- 0.2 and 139 +/- 4 torr for hemoglobin gels prepared in the absence and presence of the strong allosteric effectors inositol hexaphosphate and bezafibrate, respectively. Both values are in excellent agreement with those found for the binding of the first oxygen to hemoglobin in solution under similar experimental conditions. The corresponding Hill coefficients of hemoglobin gels were 0.94 +/- 0.02 and 0.93 +/- 0.03, indicating, in the frame of the Monod, Wyman, and Changeux model, that high and low oxygen-affinity tertiary T-state conformations have been isolated in a pure form. The values, slightly lower than unity, reflect the different oxygen affinity of alpha- and beta-hemes. Significantly, hemoglobin encapsulated in the presence of the weak effector phosphate led to gels that show intermediate oxygen affinity and Hill coefficients of 0.7 to 0.8. The heterogeneous oxygen bindin...Continue Reading

References

Jul 1, 1975·Quarterly Reviews of Biophysics·R G ShulmanS Ogawa
Jun 1, 1978·European Journal of Biochemistry·C F PoyartB Bohn
Apr 5, 1979·Journal of Molecular Biology·J Baldwin, C Chothia
Jan 1, 1975·Annual Review of Biochemistry·S J Edelstein
Sep 20, 1992·Journal of Molecular Biology·D J AbrahamJ Panikker
Jan 3, 1992·Science·G K AckersM A Daugherty
Mar 1, 1990·Biophysical Journal·M C MardenC Poyart
Feb 13, 1990·Biochemistry·I LalezariM F Perutz
Jul 5, 1990·Journal of Molecular Biology·B LuisiN Shibayama
May 1, 1989·Quarterly Reviews of Biophysics·M F Perutz
Apr 20, 1989·Journal of Molecular Biology·B Luisi, N Shibayama
Nov 20, 1986·Journal of Molecular Biology·N ShibayamaG Miyazaki
Apr 20, 1986·Journal of Molecular Biology·A ArnoneB M Hoffman
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T R Lindstrom, C Ho
Sep 1, 1971·Experimental Cell Research·J A Dvorak, W F Stotler
Jan 5, 1984·Nature·A BrzozowskiD Vallely
Apr 21, 1995·Journal of Molecular Biology·J S KavanaughR W Noble
Mar 23, 1993·Biochemistry·C RivettiW A Eaton
Mar 8, 1996·Journal of Molecular Biology·M PaoliG Dodson
Feb 1, 1997·Protein Science : a Publication of the Protein Society·A MozzarelliE R Henry
Jan 31, 1998·The Journal of Biological Chemistry·S BettatiR W Noble
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·M F PerutzG G Dodson
Apr 14, 1999·Nature Structural Biology·W A EatonA Mozzarelli
Oct 16, 1999·The Journal of Biological Chemistry·L J Juszczak, J M Friedman
May 4, 2000·Protein Science : a Publication of the Protein Society·S BrunoE R Henry
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

❮ Previous
Next ❯

Citations

Jul 22, 2009·European Journal of Applied Physiology·Hongtao XieThomas Jue
Jul 20, 2002·Biophysical Chemistry·Eric R HenryWilliam A Eaton
Mar 23, 2006·Journal of the American Chemical Society·Aaron M MassariMichael D Fayer
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cristiano ViappianiWilliam A Eaton
Jun 19, 2002·Protein Science : a Publication of the Protein Society·Robert W NobleAndrea Mozzarelli
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Giuseppe ChiricoAndrea Mozzarelli
Aug 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cristiano ViappianiWilliam A Eaton
Dec 17, 2009·Analytical Biochemistry·Zhibin MaiXiaoyong Zou
Jun 5, 2013·Physical Chemistry Chemical Physics : PCCP·Stefania AbbruzzettiCristiano Viappiani
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Martin K SafoJ Eric Russell
Jan 4, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ria Sanyal, Ambika Bhagi-Damodaran
Sep 21, 2006·The Journal of Biological Chemistry·Camille J RocheJoel M Friedman
Mar 11, 2015·Biochemistry. Biokhimii︠a︡·Qinyi Zhao
Sep 22, 2017·The Journal of Biological Chemistry·Naoya ShibayamaSam-Yong Park
Apr 2, 2005·Protein Science : a Publication of the Protein Society·Barbara CampaniniStefano Bettati
Mar 17, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Stefano BrunoAndrea Mozzarelli
Sep 3, 2013·The FEBS Journal·Marialaura MarchettiAndrea Mozzarelli
Jul 11, 2006·Protein Science : a Publication of the Protein Society·Luca RondaStefano Bettati
Sep 12, 2008·The Journal of Physical Chemistry. B·Luca RondaCristiano Viappiani
May 27, 2008·Biochimica Et Biophysica Acta·Luca RondaStefano Bettati
Oct 15, 2008·Journal of Theoretical Biology·Alfonsas Juska
Jul 21, 2006·The Journal of Physical Chemistry. B·Silvia SottiniAndrea Mozzarelli
Apr 14, 2009·Biochimica Et Biophysica Acta·Stefano BettatiAndrea Mozzarelli
Nov 3, 2010·Biochimica Et Biophysica Acta·Alessio Peracchi, Andrea Mozzarelli
Dec 28, 2010·Biochimica Et Biophysica Acta·Luca RondaAndrea Mozzarelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.