High C-X-C motif chemokine 5 expression is associated with malignant phenotypes of prostate cancer cells via autocrine and paracrine pathways

International Journal of Oncology
Ya-Ling QiWeiqun Wang

Abstract

The present study aimed to examine the effects and mechanisms of exogenous C-X-C motif chemokine 5 (CXCL5) and lentiviral CXCL5 overexpression on the regulation of malignant behaviors of prostate cancer cells in vitro and in a nude mouse xenograft model. The expression levels of CXCL5 and a number of tumor-related genes were assessed by using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, ELISA, or immunohistochemistry in normal and cancerous prostate cells and tissues. Cell proliferation, colony formation, and Transwell assays were performed to determine the effects of exogenous, autocrine, and paracrine CXCL5 on prostate cancer cell proliferative and migratory capacity. The results indicated that CXCL5 expression was upregulated in PC‑3 and DU145 prostate cancer cells, in WPMY‑1 normal prostate stromal cells, and in RWPE‑1 prostate epithelial cells, as well as in prostate cancer tissue specimens. Exogenous CXCL5 exposure resulted in increase in prostate cancer cell proliferation, colony formation, and migration. In cells transfected with a CXCL5 overexpression vector, in cells cultured in conditioned medium from CXCL5-overexpressing WPMY cells, and in cells co-cultured with CXCL5...Continue Reading

References

Apr 12, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T PerssonA Egesten
Feb 28, 2004·Expert Reviews in Molecular Medicine·Michael R DouglasChristopher D Buckley
Jul 2, 2004·Nature Reviews. Cancer·Fran Balkwill
Mar 3, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Barrett J Rollins
May 5, 2007·Journal of Cancer Research and Clinical Oncology·Jeong Youp ParkSi Young Song
Mar 6, 2008·Neoplasia : an International Journal for Oncology Research·Lesa A BegleyJill A Macoska
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frank M SpeetjensJ Fred Nagelkerke
Nov 1, 2008·International Journal of Cancer. Journal International Du Cancer·Weiqun WangRunsheng Li
Jan 6, 2009·Nature·Alberto Mantovani
Jun 27, 2009·Endocrine-related Cancer·David VindrieuxGwendal Lazennec
Dec 17, 2009·Cytokine & Growth Factor Reviews·Alberto MantovaniRaffaella Bonecchi
Mar 2, 2011·The American Journal of Pathology·Aihua LiO Joe Hines
Dec 27, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Mikio KawamuraMasato Kusunoki
Jun 20, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shao-Lai ZhouJian Zhou
Dec 19, 2013·International Journal of Cancer. Journal International Du Cancer·Po-Lin KuoYa-Ling Hsu
Jun 19, 2014·Molecular Cancer Research : MCR·Takamasa UekitaRyuichi Sakai
Jul 12, 2014·Journal of Translational Medicine·Xiaojing XuJinglin Xia
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 17, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Sima HajiahmadiMahdi Shabani
Jun 6, 2015·Biochemical and Biophysical Research Communications·Yong BianLin Li
Oct 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xi ZhuYe Tian
Feb 4, 2016·International Urology and Nephrology·Shi-Liang GuiWei-Qun Wang
Apr 16, 2016·International Journal of Oncology·Kuangfa LiTingmei Chen
Feb 7, 2018·The Medical Clinics of North America·William J Catalona

❮ Previous
Next ❯

Citations

Sep 10, 2020·Frontiers in Oncology·John D PattersonAnaliz Rodriguez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.