PMID: 8598545Mar 1, 1996Paper

High carbohydrate diet and starvation regulate lipogenic mRNA in rats in a tissue-specific manner

The Journal of Nutrition
T S Kim, H C Freake

Abstract

We have previously shown that the effects of a high carbohydrate, fat-free diet and 24-h starvation on fatty acid synthesis in rats are tissue specific. In the present study we examine the tissue-specific pretranslational effects of high carbohydrate feeding, starvation and refeeding a high carbohydrate diet after starvation on the lipogenic pathway by measuring the levels of mRNA encoding acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) using Northern analysis. Additionally, we measured mRNA S14, a sequence tightly associated with lipogenesis. In rats fed the high carbohydrate diet, hepatic levels of the three mRNA were 3-5 fold higher than in controls. The level of S14 mRNA was doubled in epididymal fat, but other effects of this diet in adipose tissues were not significant. Expression in kidney, heart, lung and brain was not altered. Starvation significantly reduced the level of these mRNA in all tissues examined except brain. In liver, refeeding the high carbohydrate diet induced the expression of ACC, FAS and S14 mRNA 20-30 fold compared with the values found in 48-h starved animals. Hyperinduction of ACC and FAS, but not S14 mRNA expression was also observed in adipose tissues. The tissue-specific nature of thes...Continue Reading

Citations

Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D G FongA L Buchman
Aug 19, 2007·The British Journal of Nutrition·Christopher J McNeilWilliam D Rees
Nov 8, 2013·European Journal of Nutrition·Alessandra FerramoscaVincenzo Zara
Apr 8, 1998·The American Journal of Physiology·F B Hillgartner, T Charron
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Nov 3, 2009·Endocrinology·Kartik ShankarThomas M Badger
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Oct 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Eun-Kyoung KimGabriele V Ronnett
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Feb 4, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sami DridiJohan Buyse
Aug 5, 2003·Journal of Lipid Research·Dominique LetexierMichel Beylot
Jan 1, 1997·Annual Review of Nutrition·K H Kim
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May 16, 2014·Advances in Nutrition·Alessandra Ferramosca, Vincenzo Zara
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Mar 5, 2021·Molecules and Cells·Jean-Mathieu Berger, Young-Ah Moon
May 5, 2009·The Journal of Experimental Biology·Chunchun HanZhixiong Pan
Nov 16, 2004·Biochemical and Biophysical Research Communications·Fabrice Bertile, Thierry Raclot
Apr 17, 1999·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·B MittendorferL S Sidossis

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