High-cell-density cultivation of Schizochytrium sp. in an ammonium/pH-auxostat fed-batch system

Biotechnology Letters
E GanuzaC Ratledge

Abstract

Thraustochytrids, in particular Schizochytrium spp., are used for the production of the valuable polyunsaturated fatty acid, docosahexaenoic acid (DHA; 22:6 n-3). Growth of Schizochytrium sp. G13/2S in a defined medium was initially made in shake-flask cultures to determine the optimum concentrations of glucose (100-200 g l(-1)) and ammonia ( approximately 300 mg l(-1)) that could be used by this microorganism. In subsequent fermenter cultures, a pH-auxostat method was used to maintain NH(3) from 200-300 mg l(-1). During the first 49 h of fermentation, 150 g glucose l(-1) produced 63 g cell dry wt l(-1). Although growth was not limited by the supply of nitrogen, total fatty acids were at 25% cell dry wt which is more than half the final lipid content of commercially-grown Schizochytrium biomass which uses N-limited medium in the final stages for maximum lipid accumulation. This strategy is therefore useful for the cultivation of Schizochytrium to a high cell density up to the point when lipid accumulation can be triggered by N exhaustion.

References

Sep 19, 2002·Advances in Applied Microbiology·Colin Ratledge, James P Wynn
Jan 17, 2003·Biotechnology and Bioengineering·Martin E De SwaafJack T Pronk
Jan 24, 2004·Applied Microbiology and Biotechnology·L Sijtsma, M E de Swaaf
May 24, 2005·Bone Marrow Transplantation·E MazzolariL D Notarangelo
Aug 19, 2007·Applied Microbiology and Biotechnology·E Ganuza, M S Izquierdo

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Citations

May 7, 2010·Applied Microbiology and Biotechnology·Lu-Jing RenPing-Kai Ouyang
Mar 13, 2009·Bioprocess and Biosystems Engineering·Lu-Jing RenXiao-Jun Ji
Sep 24, 2013·Bioprocess and Biosystems Engineering·Lu-Jing RenHe Huang
Jun 14, 2014·Bioresource Technology·Giorgos MarkouKoenraad Muylaert
Apr 29, 2015·International Journal of Molecular Sciences·Elia Judith MartínezXiomar Gómez
Dec 5, 2012·Bioresource Technology·Hong-Wei YenJo-Shu Chang
Aug 25, 2015·Bioresource Technology·Panyawut JanthanomsukSomchai Chauvatcharin
Oct 26, 2010·Water Research·Octavio Perez-GarciaYoav Bashan
Dec 10, 2008·Biochimie·John L Harwood, Irina A Guschina
Mar 13, 2012·Biotechnology Advances·Adarsha GuptaMunish Puri
Sep 20, 2020·Biotechnology and Bioengineering·Oleksandra SavchenkoJie Chen
Apr 7, 2018·Bioresource Technology·Kang WangWeiwen Zhang

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