High-cell-density fermentation for ergosterol production by Saccharomyces cerevisiae

Journal of Bioscience and Bioengineering
Fei ShangTianwei Tan

Abstract

The direct feedback control of glucose using an on-line ethanol concentration monitor for ergosterol production by high-cell-density fermentation was investigated and the fermentation parameters (e.g., pH, dissolved oxygen, ethanol concentration, oxygen uptake rate, carbon dioxide evolution rate and respiratory quotient) were analyzed. Controlling glucose feeding rate in accordance with ethanol concentration and adjusting pH with ammonia during the fermentation process were effective fed-batch methods for ergosterol production. The fermentation parameters well described the variation of the whole fermentation process. Cultivation in a 5 l fermentor was carried out under the following conditions: culture temperature, 30 degrees C; pH, 5.5; agitation speed, 600 rpm; fermentation time, 60 h; controlling ethanol concentration below 1% and keeping respiratory quotient (RQ) at approximately 1.0. Under these conditions, the yeast dry weight reached 120 g/l and the ergosterol yield reached 1500 mg/l.

References

Jan 1, 1995·Annual Review of Microbiology·L W Parks, W M Casey
Feb 5, 1992·Biotechnology and Bioengineering·G M O'ConnorC L Cooney

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Citations

Jul 25, 2008·Applied Biochemistry and Biotechnology·Lei ZhaoTianwei Tan
Oct 22, 2008·Applied Biochemistry and Biotechnology·Feiyan XueTianwei Tan
Jan 17, 2007·Applied Microbiology and Biotechnology·Xiuping HeBorun Zhang
Oct 15, 2010·Biotechnology Progress·Astrid EdlichRainer Krull
Apr 10, 2013·Progress in Lipid Research·Tamara Wriessnegger, Harald Pichler
Feb 4, 2015·World Journal of Microbiology & Biotechnology·Gabriela ContrerasJennifer Alcaíno
Dec 21, 2013·Biotechnology and Applied Biochemistry·Xiao Ying XuHong Xu
Feb 5, 2019·Biotechnology and Applied Biochemistry·Xiao LiNian Zheng
Jun 22, 2019·World Journal of Microbiology & Biotechnology·Jun-Feng LiuLi Deng

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