High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor

Journal of Biotechnology
Ingo KnabbenJochen Büchs

Abstract

A common method to minimize overflow metabolism and to enable high cell-density is to operate microbial processes in fed-batch mode under carbon-limiting conditions. This requires sophisticated process control schemes with expensive hardware equipment and software and well-characterized processes parameters. To generate high-cell density, a more simplified strategy would be beneficial. Therefore, a genetically engineered Escherichia coli strain with a modified glucose uptake system was cultivated in batch mode. In the applied strain, the usual phosphotransferase system of a K12-derived strain was inactivated, while the galactose permease system was amplified. Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L(-1), even when the batch fermentation was started with a glucose concentration of 130 g L(-1). Finally, maximum biomass concentrations of 48 g L(-1) dry cell weight and maximum space-time yields of 2.10 g L(-1) h(-1) were reached. To provide an unlimited growth under fully aerobic conditions (DOT>30%) at comparatively low values for specific power input (3-4 kW m(-3)), a pressurized bioreactor was used. Consequentially, to our knowledge, this study using ...Continue Reading

Citations

Dec 15, 2012·Bioprocess and Biosystems Engineering·Marlene LopesManuel Mota
Mar 30, 2013·Microbial Cell Factories·Stéphanie FollonierMaría Auxiliadora Prieto
Sep 10, 2013·Journal of Bioscience and Bioengineering·Martin WunderlichAlvaro R Lara
Apr 30, 2014·Biotechnology Progress·Marlene LopesManuel Mota
Dec 10, 2013·Trends in Biotechnology·Jian Yu
Nov 6, 2014·Bioscience Reports·Eva UllmannOliver Spadiut
Jul 2, 2011·Journal of Biotechnology·Tania E PablosAlvaro R Lara
Jul 5, 2016·Journal of Biotechnology·Fabiola Islas-LugoMaría Del Carmen Montes-Horcasitas
Apr 7, 2018·Applied Microbiology and Biotechnology·Yilin RenGuo-Qiang Chen
Jul 26, 2012·Biotechnology and Bioengineering·Lars RegesteinJochen Büchs
Nov 16, 2019·New Biotechnology·Lucile GarriguesStéphane E Guillouet

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