High CO2 alters the hypoxia response of the Pacific whiteleg shrimp (Litopenaeus vannamei) transcriptome including known and novel hemocyanin isoforms

Physiological Genomics
Jillian G JohnsonKaren G Burnett

Abstract

Acclimation to low O2 in many organisms involves changes at the level of the transcriptome. Here we used high-throughput RNA sequencing (RNA-Seq) to explore the global transcriptomic response and specific involvement of a suite of hemocyanin (Hc) subunits to low O2 alone and in combination with high CO2, which naturally co-occurs with low O2. Hepatopancreas mRNA of juvenile L. vannamei exposed to air-saturated water, low O2, or low O2/high CO2 for 4 or 24 h was pooled, sequenced (HiSeq 2500) and assembled (Trinity: 52,190 contigs) to create a deep strand-specific reference transcriptome. Annotation of the assembly revealed sequences encoding the previously described small Hc subunit (HcS), and three full-length isoforms of the large subunit (HcL1-3). In addition to this, a previously unidentified full-length Hc subunit was discovered. Phylogenetic analysis demonstrated the subunit to be a β-type Hc subunit (denoted HcB), making this the first report of a β-type hemocyanin subunit in the Penaeoidea. RNAs of individual shrimp were sequenced; regulated genes identified from pairwise comparisons demonstrated a distinct pattern of regulation between prolonged low O2 and low O2/high CO2 treatments by GO term enrichment analysis (Roff...Continue Reading

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Citations

Jul 15, 2016·Integrative and Comparative Biology·Jillian G JohnsonKaren G Burnett
Sep 18, 2016·Integrative and Comparative Biology·Donald L MyklesJonathon H Stillman
Aug 9, 2017·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Lillian R McCormick, Lisa A Levin
Jun 22, 2017·Insect Molecular Biology·S FlachsbarthT Burmester
Dec 13, 2016·Integrative and Comparative Biology·Sunetra DasDavid S Durica
Nov 18, 2018·Scientific Reports·Digang ZengXiaohan Chen

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