High concentration solubility and stability of ɛ-poly-l-lysine in an ammonium-based ionic liquid: A suitable media for polypeptide packaging and biomaterial preparation

International Journal of Biological Macromolecules
Rosy Alphons SequeiraKamalesh Prasad

Abstract

Packaging of structurally sensitive biomolecules such as proteins, peptides and DNA in non-aqueous media at ambient conditions with chemical and structural stability is important to explore the potential of such biomacromolecules as substrate for functional biomaterial design and for biotechnological applications. In this perspective, solubility, chemical and structural stability of ɛ-poly-l-lysine (ɛ-PL), a homopolypeptide produced by Streptomyces albulus in different ionic liquids (ILs) namely 2-hydroxyethyl ammonium formate (2-HEAF), 2-hydroxyethyl ammonium acetate (2-HEAA), choline formate (Ch-Formate) and choline acetate (Ch-Acetate) was studied. Maximum solubility (15% w/v) of the homopolypeptide was observed in 2-HEAF and lowest was found in Ch-Formate (2% w/v). After regeneration of the dissolved polypeptide in the IL, the IL could be recycled and reused in the dissolution process. Unlike in other ILs, 3-15% w/v of ɛ-PL in 2-HEAF gave formation of a thixotropic thermoreversible soft gel. Molecular docking studies established favourable interactions of [2-HEA]+ cation over [Ch]+ with ɛ-PL indicating [2-HEA]+ as the most promising cation for the dissolution process. However, the role of the anions was also found to be imp...Continue Reading

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Citations

Jan 22, 2021·Nucleic Acids Research·Ksenia S EgorovaValentine P Ananikov
May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nishith A ChudasamaKamalesh Prasad
Jul 11, 2021·Advanced Science·Alexander M CurreriEden E L Tanner

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