High concentrations of glucose reduce the oxidative metabolism of dog neutrophils in vitro

BMC Veterinary Research
A M BoscoP C Ciarlini


Dogs are commonly affected by hyperglycemic conditions. Hyperglycemia compromises the immune response and favors bacterial infections; however, reports on the effects of glucose on neutrophil oxidative metabolism and apoptosis are conflicting in humans and rare in dogs. Considering the many complex factors that affect neutrophil oxidative metabolism in vivo, we investigated in vitro the specific effect of high concentrations of glucose on superoxide production and apoptosis rate in neutrophils from healthy dogs. The capacity of the neutrophils to reduce tetrazolium nitroblue decreased significantly in the higher concentration of glucose (15.13 ± 9.73% (8 mmol/L) versus 8.93 ± 5.71% (16 mmol/L)). However, there were no changes in tetrazolium nitroblue reduction at different glucose concentrations when the neutrophils were first activated with phorbol myristate acetate. High concentrations of glucose did not affect the viability and apoptosis rate of canine neutrophils either with or without prior camptothecin stimulation. This study provides the first evidence that high concentrations of glucose inhibit the oxidative metabolism of canine neutrophils in vitro in a manner similar to that which occurs in humans, and that the decrea...Continue Reading


Nov 1, 1996·Journal of Diabetes and Its Complications·S FukaseP F Kador
Aug 18, 2000·The Journal of Clinical Endocrinology and Metabolism·P MohantyP Dandona
Nov 7, 2002·American Journal of Physiology. Renal Physiology·Barinder P S KangLeonard G Meggs
Jan 29, 2003·Intensive Care Medicine·A PernerJ Rask-Madsen
Jul 9, 2004·Journal of Leukocyte Biology·Mark T Quinn, Katherine A Gauss
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea M VincentEva L Feldman
Mar 26, 2005·Journal of Dental Research·D T GravesE Krall
Jul 29, 2005·Cell Biochemistry and Biophysics·Dana M Niedowicz, David L Daleke
Feb 8, 2006·The Journal of Endocrinology·T C Alba-LoureiroT C Pithon-Curi
Feb 24, 2006·Toxicology and Applied Pharmacology·Anabela P Rolo, Carlos M Palmeira
Apr 25, 2006·Surgical Infections·Matthias TurinaHiram C Polk
May 23, 2006·Metabolism: Clinical and Experimental·Vladimíra FejfarováTerezie Pelikánová
Aug 21, 2007·Diabetes Research and Clinical Practice·S BilgicG Deniz
Oct 18, 2007·Journal of Veterinary Internal Medicine·Danna M TorreDaniel L Chan
Feb 23, 2008·Diabetic Medicine : a Journal of the British Diabetic Association·M E StegengaT van der Poll
Apr 9, 2008·Journal of Leukocyte Biology·Kazuhiro OmoriThomas E Van Dyke
Sep 3, 2009·World Journal of Gastroenterology : WJG·Shinji KawahitoShuzo Oshita
Oct 22, 2009·American Journal of Physiology. Endocrinology and Metabolism·Viorica IonutRichard N Bergman
May 1, 2008·Journal of Indian Society of Periodontology·Neetha ShettyAmita Ramesh
May 21, 2010·Veterinary Parasitology·Pablo Gómez-OchoaJuan Antonio Castillo
Jun 1, 2010·Biochemical Pharmacology·Jamel El-BennaAxel Périanin
Jun 12, 2012·Immunity & Ageing : I & a·Kazumasa Tsukamoto, Kazuhiko Machida

Methods Mentioned


Related Concepts

Dog Diseases
Canis familiaris
Hyperglycemia, Postprandial
Neutrophil Band Cells
Apoptosis, Intrinsic Pathway
Bacterial Infections
Cell Survival

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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