High content analysis at single cell level identifies different cellular responses dependent on nanomaterial concentrations

Scientific Reports
Bella B ManshianStefaan J Soenen

Abstract

A mechanistic understanding of nanomaterial (NM) interaction with biological environments is pivotal for the safe transition from basic science to applied nanomedicine. NM exposure results in varying levels of internalized NM in different neighboring cells, due to variances in cell size, cell cycle phase and NM agglomeration. Using high-content analysis, we investigated the cytotoxic effects of fluorescent quantum dots on cultured cells, where all effects were correlated with the concentration of NMs at the single cell level. Upon binning the single cell data into different categories related to NM concentration, this study demonstrates, for the first time, that quantum dots activate both cytoprotective and cytotoxic mechanisms, resulting in a zero net result on the overall cell population, yet with significant effects in cells with higher cellular NM levels. Our results suggest that future NM cytotoxicity studies should correlate NM toxicity with cellular NM numbers on the single cell level, as conflicting mechanisms in particular cell subpopulations are commonly overlooked using classical toxicological methods.

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Citations

Dec 17, 2016·Chemical Research in Toxicology·Bella B ManshianStefaan J Soenen
Nov 26, 2016·Advanced Healthcare Materials·Bella B ManshianStefaan J Soenen
Jul 28, 2016·Lab on a Chip·Carlota A Cunha-MatosMichele Zagnoni
Aug 24, 2018·Scientific Reports·Samira FilaliPierre Miossec
Nov 2, 2018·Journal of Nanobiotechnology·Bella B ManshianStefaan J Soenen
Jan 16, 2021·Chemical Reviews·Mukaddes IzciStefaan J Soenen

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Methods Mentioned

BETA
transgenic

Software Mentioned

QDot

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