High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro

Food Chemistry
Daofeng QuJianzhong Han

Abstract

Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and mechanisms of damage in HepG2 cells using High Content Analysis (HCA). We used the CCK-8 assay to determine cell viability, providing an experimental basis for determining the safety of food additives. All of the food additives tested were observed to decrease the growth of HepG2 cells in a dose-dependent manner. Sunset yellow and sodium sulfite had IC50 values of 1.06, and 0.30g/L at 24h, respectively. HCA showed that both sunset yellow and sodium sulfite had synergistic effects on cell number, membrane permeability, mitochondrial membrane potential, intracellular calcium level, oxidative stress, and high dose group DNA damage.

Citations

Jul 8, 2020·Molecular and Cellular Biochemistry·Xu HanKeping Chen
Oct 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiongyin FanAnlong Xu
Jan 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sheng FangLiping Wang
Nov 22, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Teresa Donato, Laia Tolosa
Apr 11, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G A ChappellS J Borghoff
Jul 15, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Siyan CheYanbin Yao

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