High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer

Journal of Biomolecular Screening
Cong LiJiasheng Zheng

Abstract

Liver cancer is one of the most lethal cancer types in humans, but our understanding of the molecular mechanisms underlying this process remains insufficient. Here, we conducted high-content screening of the potential genes involved in liver cancer metastasis, which we selected from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, based on the SAMcell method and RNA interference technology. We identified two powerful genes in the liver cancer metastasis process, AEG-1 and AKR1C2, both of which proved to be positive regulators in promoting metastasis in liver cancer. Further clinical results verified their roles in liver cancer. In summary, these findings could provide new insight into the liver cancer mechanism and potentially therapeutic novel targets for liver cancer therapies in the future.

References

Nov 21, 2007·Methods in Molecular Biology·Kiyoko F Aoki-Kinoshita, Minoru Kanehisa
Jun 12, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Hanshuo ZhangJianzhong Jeff Xi
Aug 21, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F E Vera-Badillo, I F Tannock
Jul 11, 2014·Oncology Letters·Yong Huang, LE-Ping Li
Jan 27, 2015·Methods in Molecular Biology·Sean SpieringErik Willems

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Methods Mentioned

BETA
chip
PCR
transfection
scraping
chips

Software Mentioned

SAMcell
SAMcell Cell Microarray
Image Xpress

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