High content image analysis reveals function of miR-124 upstream of Vimentin in regulating motor neuron mitochondria

Scientific Reports
Tal YardeniEran Hornstein

Abstract

microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function. We further demonstrated that the intermediate filament Vimentin is a key target of miR-124 in this system. Our data establishes a new pathway for control of mitochondria function in motor neurons, revealing the value of a neuron-specific miRNA gene as a mechanism for the re-shaping of otherwise ubiquitously-expressed intermediate filament network, upstream of mitochondria activity and cellular metabolism.

References

Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H S Penefsky
May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
May 9, 2006·Experimental Gerontology·Susanne HauptmannWalter E Müller
Nov 23, 2006·Nature Reviews. Neuroscience·Kenneth S Kosik
Feb 3, 2007·The New England Journal of Medicine·J Timothy Greenamyre
Nov 4, 2008·Nature Methods·Stijn van DongenAnton J Enright
Nov 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Armaz AschrafiBarry B Kaplan
Dec 17, 2008·Neuron·Mark P MattsonAiwu Cheng
Jun 19, 2009·Nature·Sung Wook ChiRobert B Darnell
Nov 6, 2009·Nature Reviews. Neuroscience·Gerhard Schratt
Dec 8, 2009·Nature Structural & Molecular Biology·Mayuko YodaYukihide Tomari
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sharon HaramatiEran Hornstein
May 13, 2011·Molecular Biology of the Cell·Oxana E NekrasovaAlexander A Minin
May 18, 2011·The Journal of Biological Chemistry·Lue HuangDominique Weil
May 24, 2011·Journal of Neurology·Massimiliano FilostoAlessandro Padovani
Jun 4, 2011·PloS One·Eric BarreyXavier Gidrol
Jun 23, 2011·PloS One·Simonetta BandieraAlexandra Henrion-Caude
Jul 15, 2011·Nature·Andrew S YooGerald R Crabtree
Aug 11, 2011·Progress in Neurobiology·Mauro Cozzolino, Maria Teresa Carrì
Sep 14, 2011·Methods in Molecular Biology·Carol Milligan, David Gifondorwa
Jan 6, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jordi MagranéGiovanni Manfredi
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michele CarrerEric N Olson
Apr 2, 2013·Molecular Medicine & Therapeutics·Xuejun H ParsonsDennis A Moore
Jun 7, 2013·Human Molecular Genetics·Erica D KovalTimothy M Miller
Sep 11, 2013·Biochimica Et Biophysica Acta·Marco TomasettiLanfeng Dong
Dec 5, 2013·Molecular Therapy. Nucleic Acids·Ravi L RungtaBrian A Macvicar
Apr 4, 2014·Journal of Molecular Neuroscience : MN·Katie NolanJochen H M Prehn
May 3, 2014·Molecular and Cellular Neurosciences·Victoria M HoKelsey C Martin

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Citations

Oct 22, 2020·Cellular and Molecular Life Sciences : CMLS·Paresh Kumar Purohit, Neeru Saini
Nov 11, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Efthalia AngelopoulouChristina Piperi
Feb 13, 2021·Cellular and Molecular Life Sciences : CMLS·Mitali ShahVaishnavi Ananthanarayanan
Jul 3, 2021·International Journal of Molecular Sciences·Ana Rita VazDora Brites

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Methods Mentioned

BETA
transfection
PCR
transmission electron microscopy
CLIP

Software Mentioned

imageJ
DAVID
TopHat2
TargetScan
HTSeq
Illumina CASAVA -
Sylamer
Fasta
MetaXpress Outgrowth
MetaXpress

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