High-Content Imaging and Gene Expression Approaches To Unravel the Effect of Surface Functionality on Cellular Interactions of Silver Nanoparticles

ACS Nano
Bella B ManshianStefaan J Soenen

Abstract

The toxic effects of Ag nanoparticles (NPs) remain an issue of debate, where the respective contribution of the NPs themselves and of free Ag(+) ions present in the NP stock suspensions and after intracellular NP corrosion are not fully understood. Here, we employ a recently set up methodology based on high-content (HC) imaging combined with high-content gene expression studies to examine the interaction of three types of Ag NPs with identical core sizes, but coated with either mercaptoundecanoic acid (MUA), dodecylamine-modified poly(isobutylene-alt-maleic anhydride) (PMA), or poly(ethylene glycol) (PEG)-conjugated PMA with two types of cultured cells (primary human umbilical vein endothelial cells (HUVEC) and murine C17.2 neural progenitor cells). As a control, cells were also exposed to free Ag(+) ions at the same concentration as present in the respective Ag NP stock suspensions. The data reveal clear effects of the NP surface properties on cellular interactions. PEGylation of the NPs significantly reduces their cellular uptake efficiency, whereas MUA-NPs are more prone to agglomeration in complex tissue culture media. PEG-NPs display the lowest levels of toxicity, which is in line with their reduced cell uptake. MUA-NPs di...Continue Reading

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Citations

Feb 2, 2016·Toxicology and Applied Pharmacology·Christian RiebelingAndrea Haase
Jan 20, 2016·Nanoscale Research Letters·Vira KravetsAnatoliy Pinchuk
Aug 31, 2016·Journal of Colloid and Interface Science·Joanna RejmanWolfgang J Parak
Nov 26, 2016·Advanced Healthcare Materials·Bella B ManshianStefaan J Soenen
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Jun 18, 2017·Journal of Nanobiotechnology·Bella B ManshianStefaan J Soenen
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Nov 2, 2018·Journal of Nanobiotechnology·Bella B ManshianStefaan J Soenen
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Mar 3, 2021·ACS Nano·Carlos Sanchez-CanoWolfgang J Parak
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Dec 17, 2016·Chemical Research in Toxicology·Bella B ManshianStefaan J Soenen

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