High-content screening data management for drug discovery in a small- to medium-size laboratory: results of a collaborative pilot study focused on user expectations as indicators of effectiveness

Journal of Laboratory Automation
Cynthia A BerlinickeG Steven Bova

Abstract

High-content screening (HCS) technology provides a powerful vantage point to approach biological problems; it allows analysis of cell parameters, including changes in cell or protein movement, shape, or texture. As part of a collaborative pilot research project to improve bioscience research data integration, we identified HCS data management as an area ripe for advancement. A primary goal was to develop an integrated data management and analysis system suitable for small- to medium-size HCS programs that would improve research productivity and increase work satisfaction. A system was developed that uses Labmatrix, a Web-based research data management platform, to integrate and query data derived from a Cellomics STORE database. Focusing on user expectations, several barriers to HCS productivity were identified and reduced or eliminated. The impact of the project on HCS research productivity was tested through a series of 18 lab-requested integrated data queries, 7 of which were fully enabled, 7 partially enabled, and 4 enabled through data export to standalone data analysis tools. The results are limited to one laboratory, but this pilot suggests that through an "implementation research" approach, a network of small- to medium...Continue Reading

References

Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Nov 30, 2004·Chembiochem : a European Journal of Chemical Biology·Zachary E PerlmanThomas U Mayer
Nov 3, 2006·The Journal of Clinical Investigation·Pedro Cuatrecasas
Nov 7, 2006·International Journal of Medical Informatics·Nicholas R AndersonPeter Tarczy-Hornoch
Apr 27, 2007·Journal of the American Medical Informatics Association : JAMIA·Nicholas R AndersonPeter Tarczy-Hornoch
Mar 1, 2008·Journal of the American Medical Informatics Association : JAMIA·Joan S AshPeter Tarczy-Hornoch
May 7, 2009·Annual Review of Biophysics·Jason R SwedlowUNKNOWN OME Consortium
Sep 22, 2009·Nature Methods·Lit-Hsin LooSteven J Altschuler
Jun 15, 2010·International Journal of Information Management·Sahiti Myneni, Vimla L Patel
Jul 14, 2010·Journal of Biomolecular Screening·Karol KozakGabor Csucs
Jul 20, 2010·Journal of Biomolecular Screening·D Lansing Taylor
Jul 20, 2010·Journal of Biomolecular Screening·Donald JacksonJames Gill

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
dissection

Software Mentioned

Cellomics Neuronal Profiling
BioFortis
Cellomics
HCS Explorer
Partek Genomics Suite
BioFortis Labmatrix
SQL Server
Cellomics STORE
HCS Discover
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.