High content screening in chemical biology: overview and main challenges

Médecine sciences : M/S
Priscille BrodinEmmanuelle Soleilhac

Abstract

The last two decades have seen the development of high content screening (HCS) methodology and its adaptation for the evaluation of small molecules as drug candidates or their use as chemical tools for research purpose. HCS was initially set-up for the understanding of the mechanism of action of compounds by testing them on cell based-assays for pharmacological and toxicological studies. Since the last decade, the use of HCS has been extended to academic research laboratories and this technology has become the starting point for numerous projects aiming at the identification of molecular targets and cellular pathways for a given disease on which novel type of drugs could act. This screening approach relies on image capture of fluorescently labeled cells therefore generating a large amount of data that must be handled by appropriate automated image analysis methods and storage instrumentation. These latter in addition to the integration and data sharing are current challenges that HCS must still tackle.

References

Jan 9, 2004·Journal of Biomolecular Screening·Christine BrideauAndy Liaw
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kimberly L CareyGary E Ward
Aug 3, 2004·Nature Reviews. Drug Discovery·Ismail Kola, John Landis
Nov 13, 2004·Science·Zachary E PerlmanSteven J Altschuler
Dec 18, 2004·Methods in Cell Biology·Randall T Peterson, Mark C Fishman
Aug 17, 2005·Journal of Biomolecular Screening·Dmytro Kevorkov, Vladimir Makarenkov
Feb 9, 2006·Nature Biotechnology·Nathalie MaloRobert Nadon
Apr 22, 2006·Nature Methods·Beate NeumannJan Ellenberg
May 10, 2006·Current Opinion in Chemical Biology·Ulrike S Eggert, Timothy J Mitchison
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Nov 10, 2006·Journal of Biomolecular Screening·Andrei GagarinPablo Zentilli
Nov 18, 2006·Methods in Enzymology·Daniel R RinesSumit K Chanda
Mar 31, 2007·BMC Bioinformatics·Nicholas A HamiltonRohan D Teasdale
Apr 3, 2007·Nature Methods·Lit-Hsin LooSteven J Altschuler
Oct 13, 2007·Current Opinion in Chemical Biology·Kerstin Korn, Eberhard Krausz
Aug 30, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·D FenisteinA Genovesio
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thouis R JonesDavid M Sabatini
Sep 22, 2009·Nature Methods·Lit-Hsin LooSteven J Altschuler
Nov 26, 2009·Combinatorial Chemistry & High Throughput Screening·Joseph M Zock
Mar 18, 2010·Nature Methods·Thomas WalterJean-Karim Hériché
May 22, 2010·Journal of Biomolecular Screening·Aabid ShariffRobert F Murphy
Feb 7, 2012·New Biotechnology·Kaylene J SimpsonPeter R Boag
Feb 14, 2012·Current Opinion in Biotechnology·Markus Rimann, Ursula Graf-Hausner
Apr 12, 2012·Drug Discovery Today·Neil O CarragherMargaret C Frame
Apr 24, 2012·Nature Methods·Carolina WählbyAnne E Carpenter
Jul 14, 2012·Expert Opinion on Drug Discovery·Daniel V LaBarberaByong Hoon Yoo
Oct 18, 2012·Drug Discovery Today·Susan Breslin, Lorraine O'Driscoll
Oct 24, 2013·Médecine sciences : M/S·Renaud PrudentLaurence Lafanechère
Dec 18, 2013·Science·Tim WangEric S Lander
Dec 18, 2013·Science·Ophir ShalemFeng Zhang
Feb 28, 2014·Médecine sciences : M/S·Barbara Dupret, Pierre-Olivier Angrand
Mar 20, 2014·Journal of Biomolecular Screening·Kevin Smith, Peter Horvath
Dec 30, 2014·Médecine sciences : M/S·Hélène Gilgenkrantz
Mar 7, 2015·Médecine sciences : M/S·Cédric S Asensio

❮ Previous
Next ❯

Citations

Aug 6, 2015·Archives of Pharmacal Research·Yue ZhuMing-Wei Wang
May 12, 2015·Médecine sciences : M/S·Caroline BaretteMarie-Odile Fauvarque
Mar 20, 2021·Médecine sciences : M/S·Preety ShabajeeJean-Philippe Stéphan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.