PMID: 9554583Apr 29, 1998Paper

High contrast and homogeneous staining of paraffin sections of whole human brains for three dimensional ultrahigh resolution image analysis

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
O Schmitt, R Eggers

Abstract

The gallocyanin chromalum stain belongs to the classical DNA-RNA staining techniques in histochemistry. It has some important features for successful object orientated image analysis of whole sections of the human brain. To obtain reproducible staining results with these large sections, the method of Einarson was adapted to image analytical requirements. We discuss staining in a warm staining solution, pH adjustment, and optimal stain composition. The embedding procedure for whole human brains is considered as well.

References

Oct 1, 1958·The Journal of Pathology and Bacteriology·T S BESWICK
Nov 1, 1960·Stain Technology·J Y TERNER, G CLARK
Jan 1, 1960·British Journal of Haematology·G H BEAVENJ C WHITE
Jul 1, 1960·Stain Technology·J Y TERNER, G CLARK
Oct 18, 1963·Zeitschrift für Zellforschung und Mikroskopische Anatomie. Abteilung Histochemie·W SANDRITTERW RICK

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Citations

May 13, 2005·Microscopy Research and Technique·Oliver SchmittJan Modersitzki

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