High-contrast fluorescence imaging based on the polarization dependence of the fluorescence enhancement using an optical interference mirror slide

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Mitsuru Yasuda, Takuo Akimoto

Abstract

High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.

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Oct 13, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mitsuru Yasuda, Takuo Akimoto

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Citations

Jun 13, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Weida WangGuowu Yang

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