High Contrast PET Imaging of GRPR Expression in Prostate Cancer Using Cobalt-Labeled Bombesin Antagonist RM26

Contrast Media & Molecular Imaging
Bogdan MitranAnna Orlova

Abstract

High gastrin releasing peptide receptor (GRPR) expression is associated with numerous cancers including prostate and breast cancer. The aim of the current study was to develop a 55Co-labeled PET agent based on GRPR antagonist RM26 for visualization of GRPR-expressing tumors. Labeling with 57Co and 55Co, stability, binding specificity, and in vitro and in vivo characteristics of 57Co-NOTA-PEG2-RM26 were studied. NOTA-PEG2-RM26 was successfully radiolabeled with 57Co and 55Co with high yields and demonstrated high stability. The radiopeptide showed retained binding specificity to GRPR in vitro and in vivo. 57Co-NOTA-PEG2-RM26 biodistribution in mice was characterized by rapid clearance of radioactivity from blood and normal non-GRPR-expressing organs and low hepatic uptake. The clearance was predominantly renal with a low degree of radioactivity reabsorption. Tumor-to-blood ratios were approximately 200 (3 h pi) and 1000 (24 h pi). The favorable biodistribution of cobalt-labeled NOTA-PEG2-RM26 translated into high contrast preclinical PET/CT (using 55Co) and SPECT/CT (using 57Co) images of PC-3 xenografts. The initial biological results suggest that 55Co-NOTA-PEG2-RM26 is a promising tracer for PET visualization of GRPR-expressin...Continue Reading

References

Jan 1, 1993·Methods in Enzymology·W Maret, B L Vallee
Oct 1, 1995·Journal of the Neurological Sciences·H M JansenJ Korf
May 8, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·M LlinaresJ Martinez
Apr 8, 2000·Nuclear Medicine and Biology·P GoethalsN Lameire
Aug 16, 2003·Endocrine Reviews·Jean Claude Reubi
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mihaela GinjJean Claude Reubi
Jan 31, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Hanwen ZhangHelmut R Maecke
Jan 18, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Renzo CescatoJean Claude Reubi
Feb 5, 2008·Chemistry : a European Journal·Axel HeppelerHelmut R Maecke
Mar 28, 2009·Nuclear Medicine and Biology·William C EckelmanChester A Mathis
May 22, 2009·AJR. American Journal of Roentgenology·Gary J KelloffUNKNOWN Prostate Cancer Imaging Working Group
Jun 27, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Helena WållbergAnna Orlova
Aug 4, 2009·Nuclear Medicine and Biology·Eftychia KoumarianouSpyridon C Archimandritis
Feb 16, 2010·Biochimica Et Biophysica Acta·Vladimir Tolmachev, Sharon Stone-Elander
Nov 15, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Keelara AbirajHelmut R Maecke
May 31, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Helge ThisgaardPoul Flemming Høilund-Carlsen
Apr 10, 2015·Physics in Medicine and Biology·P E N BraadP F Høilund-Carlsen
Nov 13, 2015·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Johan Hygum DamHelge Thisgaard
Mar 18, 2016·International Journal of Oncology·Bogdan MitranAnna Orlova

❮ Previous
Next ❯

Citations

Jan 18, 2018·Journal of Labelled Compounds & Radiopharmaceuticals·Eduardo Aluicio-SarduyJonathan W Engle
May 12, 2019·International Journal of Cancer. Journal International Du Cancer·Bogdan MitranAnna Orlova
Mar 19, 2020·International Journal of Molecular Sciences·Sara S RinneAnna Orlova
Sep 10, 2020·Nuclear Medicine and Biology·Heinz H Coenen, Johannes Ermert
Oct 10, 2018·Chemical Reviews·Eszter Boros, Alan B Packard

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
electrophoresis
xenografts

Software Mentioned

GraphPad Prism
TracerDrawer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.