High Correlation Between Structure Development and Chemical Variation During Biofilm Formation by Vibrio parahaemolyticus

Frontiers in Microbiology
Ling TanYong Zhao

Abstract

The complex three-dimensional structure of biofilms is supported by extracellular polymeric substances (EPSs) and additional insight on chemical variations in EPS and biofilm structure development will inform strategies for control of biofilms. Vibrio parahaemolyticus VPS36 biofilm development was studied using confocal laser scanning microscopy (CLSM) and Raman spectroscopy (RM). The structural parameters of the biofilm (biovolume, mean thickness, and porosity) were characterized by CLSM and the results showed that VPS36 biofilm formed dense structures after 48 h incubation. There were concurrent variations in carbohydrates and nucleic acids contents in the EPS as evidenced by RM. The Raman intensities of the chemical component in EPS, measured using Pearson's correlation coefficient, were positively correlated with biovolume and mean thickness, and negatively correlated with porosity. The Raman intensity for carbohydrates correlated closely with mean thickness (p-value < 0.01) and the Raman intensity for nucleic acid correlated closely with porosity (p-value < 0.01). Additional evidence for these correlations were confirmed using scanning electron microscopic (SEM) and crystal violet staining.

References

Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Jul 31, 2004·Analytical Chemistry·Wei E HuangAndrew S Whiteley
Apr 10, 2007·Food Microbiology·Yi-Cheng Su, Chengchu Liu
Oct 25, 2008·Analytical Chemistry·Natalia P IvlevaChristoph Haisch
Nov 4, 2008·Analytical and Bioanalytical Chemistry·Natalia P IvlevaChristoph Haisch
Jul 1, 2010·Journal of Biophotonics·Natalia P IvlevaChristoph Haisch
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
Aug 6, 2010·The Journal of Physical Chemistry. B·Natalia P IvlevaChristoph Haisch
Jul 26, 2011·Microbes and Infection·Christopher A BrobergKim Orth
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Carey D Nadell, Bonnie L Bassler
Apr 3, 2013·Cold Spring Harbor Perspectives in Medicine·Maria KostakiotiScott J Hultgren
May 7, 2013·Applied Spectroscopy·Esen Efeoglu, Mustafa Culha
Apr 20, 2014·Archives of Microbiology·Marwan AbdallahNour-Eddine Chihib
Dec 24, 2014·International Journal of Molecular Sciences·Ota SamekMartina Mahelová
Mar 11, 2016·Nature Protocols·Holly J ButlerFrancis L Martin
Jul 28, 2016·Nature Reviews. Microbiology·Carey D NadellKevin R Foster
Aug 6, 2016·International Journal of Food Microbiology·M Mosquera-FernándezE Balsa-Canto
Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Jan 17, 2017·Trends in Microbiology·Anna Dragoš, Ákos T Kovács
Jun 24, 2017·Frontiers in Microbiology·Qiao HanYong Zhao
Sep 26, 2017·Nature Reviews. Microbiology·Hyun KooLuanne Hall-Stoodley

❮ Previous
Next ❯

Citations

Oct 23, 2019·Environmental Microbiology·Henriette L RøderMette Burmølle
Nov 26, 2019·Indian Journal of Microbiology·Thanachaporn YaikhanNatta Tansila
Nov 30, 2019·TheScientificWorldJournal·Josiane Lima MendesRenata Albuquerque Costa
Dec 29, 2020·Frontiers in Cellular and Infection Microbiology·Lianzhi YangLanming Chen
Sep 22, 2019·Microorganisms·Andrea FanesiFilipa Lopes

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning
transmission electron microscopy
scraping

Software Mentioned

ISA
SPSS
Origin pro
Bruker OPUS
Zen

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.