High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest

Antioxidants
Garry ClarkeJeffrey Fry

Abstract

Extracts of plants from the Malaysian rainforest and other fragile habitats are being researched intensively for identification of beneficial biological actions, with assessment of antioxidant behavior being a common component of such assessments. A number of tests for antioxidant behavior are used, with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction activity potential (FRAP) assays often being used in parallel, and also with measurement of total phenolics content (TPC) as a surrogate marker for antioxidant capacity. The present study investigated the possible redundancy in using all three assays to determine antioxidant capacity in 92 extracts obtained from 27 plants from the Malaysian rainforest. The results demonstrated that the assays displayed a high (R ≥ 0.82) and significant (P < 0.0001) correlation with one another, indicating a high level of redundancy if all three assays are used in parallel. This appears to be a waste of potentially valuable plant extracts. Because of problems with the FRAP assay relating to color interference and variable rates of reaction point, the DPPH assay is the preferred assay in preliminary screening of extracts of plants from the Malaysian rainforest.

References

Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Dejian HuangRonald L Prior
Jun 1, 2000·Nutrition Research Reviews·G G DuthieJ A Kyle
Mar 17, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hao ZhangFazheng Ren
Nov 26, 2010·Journal of Microbiological Methods·Mukhrizah OthmanKang Nee Ting
Apr 23, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Suhailah Wasman QaderSalehhuddin Hamdan
May 10, 2011·Journal of Agricultural and Food Chemistry·Rui Fen ZhangJian Wei Chi
Jul 15, 2011·Food Chemistry·Aleksandra Szydłowska-CzerniakEdward Szłyk

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Citations

Mar 10, 2017·BMC Complementary and Alternative Medicine·Saira TabassumGul Majid Khan
Feb 13, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kristóf CsepregiÉva Hideg
Jun 17, 2015·Chembiochem : a European Journal of Chemical Biology·Alexander LepakBernd Nidetzky
May 9, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudio B S OliveiraValter F Andrade-Neto
Nov 6, 2018·Natural Product Research·Rico RamadhanPreecha Phuwapraisirisan
May 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Qiang HuangPei-Bo Li
Mar 3, 2018·Environmental Science and Pollution Research International·Anwar Hussain PhulpotoNisar Ahmed Kanhar
Nov 11, 2017·The Journal of Pharmacy and Pharmacology·Mosad Ahmed GhareebMichael Wink
Jan 20, 2018·BioMed Research International·Rehana RashidGhulam Murtaza
Aug 31, 2019·BioMed Research International·Huma TariqMingxing Zhang
Dec 15, 2015·In Vitro Cellular & Developmental Biology. Animal·Venkataraman DeepakKrishnan Sundar
Sep 18, 2014·Natural Product Research·Radia Mahboub, Faiza Memmou
Mar 24, 2018·BioMed Research International·Mohamed MakniHamadi Fetoui
Nov 28, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sheona N InnesSissel Torre
Oct 31, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Bundit PromraksaPatcharee Boonsiri
Nov 10, 2020·Journal of Pharmaceutical and Biomedical Analysis·Júlia Morais FernandesSilvana Maria Zucolotto
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Phraepakaporn KunnajaSeewaboon Sireeratawong
May 25, 2021·Biotechnology and Applied Biochemistry·Shivankar AgrawalSunil Kumar Deshmukh
Jun 3, 2021·Antioxidants·Melanie PlatzerPeter Eisner
Dec 1, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Noor Ul HudaAbdul Mannan
Jul 10, 2021·BMC Complementary Medicine and Therapies·Fiaz AlamMohammad Hassham Hassan Bin Asad
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Saima AliZartash Zahra

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