High-density 16S microarray and clone library-based microbial community composition of the Phoenix spacecraft assembly clean room

Astrobiology
Parag VaishampayanKasthuri Venkateswaran

Abstract

The bacterial diversity and comparative community structure of a clean room used for assembling the Phoenix spacecraft was characterized throughout the spacecraft assembly process by using 16S rRNA gene cloning/sequencing and DNA microarray (PhyloChip) technologies. Samples were collected from several locations of the clean room at three time points: before Phoenix's arrival (PHX-B), during hardware assembly (PHX-D), and after the spacecraft was removed for launch (PHX-A). Bacterial diversity comprised of all major bacterial phyla of PHX-B was found to be statistically different from PHX-D and PHX-A samples. Due to stringent cleaning and decontamination protocols during assembly, PHX-D bacterial diversity was dramatically reduced when compared to PHX-B and PHX-A samples. Comparative community analysis based on PhyloChip results revealed similar overall trends as were seen in clone libraries, but the high-density phylogenetic microarray detected larger diversity in all sampling events. The decrease in community complexity in PHX-D compared to PHX-B, and the subsequent recurrence of these organisms in PHX-A, speaks to the effectiveness of NASA cleaning protocols. However, the persistence of a subset of bacterial signatures throug...Continue Reading

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Citations

Feb 25, 2011·The ISME Journal·Jizhong ZhouYunfeng Yang
Oct 12, 2012·The ISME Journal·Parag VaishampayanKasthuri Venkateswaran
Jul 17, 2012·Astrobiology·Michaela StieglmeierChristine Moissl-Eichinger
Jun 26, 2012·Applied and Environmental Microbiology·Myron T La DucKasthuri Venkateswaran
Mar 15, 2011·Applied and Environmental Microbiology·K KwanK Venkateswaran
Jul 18, 2014·International Journal of Systematic and Evolutionary Microbiology·Parag VaishampayanKasthuri Venkateswaran
Oct 27, 2017·Microbiome·Kaisa KoskinenChristine Moissl-Eichinger
Oct 5, 2019·Scientific Reports·Ganesh Babu Malli MohanKasthuri Venkateswaran
Feb 20, 2021·Microbiology Resource Announcements·Velislava IlievaMichael C Macey

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Datasets Mentioned

BETA
EU704699
EU706281
AJ270258
AB008315
AB075017
AB021405

Methods Mentioned

BETA
PCR
chip
phylogenetic microarray

Software Mentioned

BLAST
Perl
DNADIST
DOTUR
Clustal W
LIBSHUFF
UniFrac
SONS
PhyloChip
PHYLIP

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