High-density lipoprotein endocytosis in endothelial cells

World Journal of Biological Chemistry
Stefanie FruhwürthHerbert Stangl

Abstract

To describe the way stations of high-density lipoprotein (HDL) uptake and its lipid exchange in endothelial cells in vitro and in vivo. A combination of fluorescence microscopy using novel fluorescent cholesterol surrogates and electron microscopy was used to analyze HDL endocytosis in great detail in primary human endothelial cells. Further, HDL uptake was quantified using radio-labeled HDL particles. To validate the in vitro findings mice were injected with fluorescently labeled HDL and particle uptake in the liver was analyzed using fluorescence microscopy. HDL uptake occurred via clathrin-coated pits, tubular endosomes and multivesicular bodies in human umbilical vein endothelial cells. During uptake and resecretion, HDL-derived cholesterol was exchanged at a faster rate than cholesteryl oleate, resembling the HDL particle pathway seen in hepatic cells. In addition, lysosomes were not involved in this process and thus HDL degradation was not detectable. In vivo, we found HDL mainly localized in mouse hepatic endothelial cells. HDL was not detected in parenchymal liver cells, indicating that lipid transfer from HDL to hepatocytes occurs primarily via scavenger receptor, class B, type I mediated selective uptake without conco...Continue Reading

Citations

Mar 11, 2018·Lipids in Health and Disease·Mónica Muñoz-VegaÓscar Pérez-Méndez
Jan 1, 2020·Journal of the American Heart Association·Bikash ManandharKerry-Anne Rye
Oct 26, 2018·Circulation Research·Xinchun PiCam Patterson
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alan Dorantes-MoralesElizabeth Carreón-Torres
Jan 28, 2017·Frontiers in Immunology·Marion HumbertJuan Dubrot
Dec 21, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Dan GiladAndrea Szuchman-Sapir
Oct 10, 2020·Atherosclerosis·Erika JangWarren L Lee
May 1, 2021·Cells·Jérôme RobertArnold von Eckardstein
May 13, 2021·Circulation Research·Sara N KoenigPeter J Mohler

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