High density lipoprotein (HDL) reverses palmitic acid induced energy metabolism imbalance by switching CD36 and GLUT4 signaling pathways in cardiomyocyte

Journal of Cellular Physiology
Su-Ying WenChih-Yang Huang

Abstract

In our previous study palmitic acid (PA) induced lipotoxicity and switches energy metabolism from CD36 to GLUT4 in H9c2 cells. Low level of high density lipoprotein (HDL) is an independent risk factor for cardiac hypertrophy. Therefore, we in the present study investigated whether HDL can reverse PA induced lipotoxicity in H9c2 cardiomyoblast cells. In this study, we treated H9c2 cells with PA to create a hyperlipidemia model in vitro and analyzed for CD36 and GLUT4 metabolic pathway proteins. CD36 metabolic pathway proteins (phospho-AMPK, SIRT1, PGC1α, PPARα, CPT1β, and CD36) were decreased by high PA (150 and 200 μg/μl) concentration. Interestingly, expression of GLUT4 metabolic pathway proteins (p-PI3K and pAKT) were increased at low concentration (50 μg/μl) and decreased at high PA concentration. Whereas, phospho-PKCζ, GLUT4 and PDH proteins expression was increased in a dose dependent manner. PA treated H9c2 cells were treated with HDL and analyzed for cell viability. Results showed that HDL treatment induced cell proliferation efficiency in PA treated cells. In addition, HDL reversed the metabolic effects of PA: CD36 translocation was increased and reduced GLUT4 translocation, but HDL treatment significantly increased CD3...Continue Reading

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W SlotG E Lienhard
Sep 1, 1972·The Journal of Clinical Investigation·L HagenfeldtL Räf
Oct 29, 1998·The Journal of Biological Chemistry·G W GoodwinH Taegtmeyer
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y T ZhouR H Unger
May 4, 2002·Heart Failure Reviews·William C Stanley, Margaret P Chandler
Apr 12, 2003·Progress in Lipid Research·Nandakumar Sambandam, Gary D Lopaschuk
Jul 28, 2004·Diabetes Care·Gregory A NicholsJonathan B Brown
Aug 18, 2004·Apoptosis : an International Journal on Programmed Cell Death·N BeeharryI C Green
Dec 2, 2004·Canadian Journal of Physiology and Pharmacology·David L Severson
Apr 14, 2006·Molecular and Cellular Biology·Qiwei WuAndreas Stahl
Dec 1, 2006·Current Opinion in Biotechnology·Omer Aras, Vasken Dilsizian
Dec 5, 2006·The Journal of Clinical Investigation·Daniel J Rader
Jul 4, 2007·Nutrition Reviews·William L HollandScott A Summers
Feb 2, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Adrian ChabowskiArend Bonen
Jun 18, 2009·American Journal of Physiology. Endocrinology and Metabolism·Louis Hue, Heinrich Taegtmeyer
Aug 25, 2009·Cardiovascular Research·Miguel A FriasRichard W James
Sep 22, 2009·European Journal of Pharmacology·Elias KatsoulierisPrabal K Chatterjee
Jan 21, 2010·Physiological Reviews·Gary D LopaschukWilliam C Stanley
Sep 10, 2010·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Nosratola D Vaziri
Jan 11, 2011·Journal of Lipid Research·May BrundertFranz Rinninger
Mar 1, 2011·Physiology & Behavior·Céline MartinPhilippe Besnard
Dec 29, 2011·Molecular and Cellular Biochemistry·Young Sik ChoHyae Gyeong Cheon
Mar 3, 2012·Circulation Research·William C StanleyKelly A O'Connell
Mar 21, 2012·Biochemical and Biophysical Research Communications·Yong-Woon KimKyu Chang Won
May 1, 2012·Annual Review of Nutrition·Xuewei Zhu, John S Parks
Dec 12, 2012·Journal of Cardiopulmonary Rehabilitation and Prevention·Phillip B SparlingJames O Hill
Jan 15, 2013·Journal of Clinical Lipidology·Hicham BerrouguiAbdelouahed Khalil
Mar 20, 2013·Mediators of Inflammation·Débora EstadellaAline de Piano

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