Abstract
To investigate the effect and mechanism of high density lipoprotein (HDL) on type II alveolar epithelial cells during inflammation state. The original generation of type II alveolar epithelial cells were separated in rats and treated with PBS/LPS/HDL/HDL + LPS. To observe the proliferation and migration of type II alveolar epithelial cells with bromodeoxyuridine(BrdU) assay, transwell assay and wound healing experiments. In addition, western blot detected the expression of TP-binding cassette transporter A1 (ABCA1), cystic fibrosis transmembrane conductance regulator (CFTR) and the phosphorylation of AKT/extracellular signal-regulated kinase(ERK)/mitogen-activated protein kinase(MAPK). Enzyme-linked immunosorbent assay (ELISA) tested the secretion of tumor necrosis factor a(TNF-a)/interleukin 1a(IL-1a)/IL-6. HDL promoted the proliferation (↑17%, p < 0.001 HDL+ LPS vs. LPS) and migration (wounding healing: ↑93%, p < 0.001 HDL+ LPS vs. LPS; transwell migration: ↑154%, p < 0.001 HDL+ LPS vs. LPS) of type II alveolar epithelial cells. Furthermore, HDL increased the phosphorylation of MAPK, but not AKT/ERK. And HDL decreased the secretion of TNF-a (↓46%, p < 0.01 HDL+ LPS vs. LPS) and IL-1a (↓45%, p < 0.001 HDL+ LPS vs. LPS), but no...Continue Reading
References
Nov 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·G W CockerillP J Barter
Sep 1, 1994·The Journal of Experimental Medicine·M M WurfelS D Wright
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D M LevineA L Rubin
Oct 9, 1999·Free Radical Biology & Medicine·I KolleckB Rüstow
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J McNeishO L Francone
Aug 12, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anna E BortnickSandra R Bates
Mar 23, 2004·American Journal of Respiratory Cell and Molecular Biology·Ian R WitherdenTeresa D Tetley
Oct 16, 2004·Circulation Research·Philip J BarterAlan M Fogelman
Aug 2, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sandra R BatesGeorge H Rothblat
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J ThorleyTeresa D Tetley
Sep 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Sandra R BatesGeorge H Rothblat
Jul 9, 2008·The Journal of Biological Chemistry·Kelley M ArgravesW Scott Argraves
Feb 28, 2009·PloS One·Neeraj VijPamela L Zeitlin
Dec 19, 2009·The Journal of Biological Chemistry·Takao KimuraFumikazu Okajima
Feb 10, 2011·Nature Reviews. Cardiology·Mohamad NavabAlan M Fogelman
Aug 2, 2011·International Journal of Cancer. Journal International Du Cancer·Bing PanLemin Zheng
Jan 21, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bing PanLemin Zheng
Mar 20, 2012·American Journal of Respiratory Cell and Molecular Biology·Cuilian DaiStewart J Levine
Jun 12, 2012·BMC Musculoskeletal Disorders·Wei XuQi Rong Dong
Oct 26, 2012·Methods in Molecular Biology·Robert F Gonzalez, Leland G Dobbs
Nov 8, 2012·PloS One·Bing PanLemin Zheng
Nov 14, 2012·Infection and Immunity·Olga D ChuquimiaCarmen Fernández
Feb 9, 2013·PloS One·Lefeng WangSanjay Mehta
May 22, 2013·PloS One·Oleg F SharifovHimanshu Gupta
Jul 31, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S-W ParkC-S Park
May 13, 2014·American Journal of Respiratory Cell and Molecular Biology·Cuilian DaiStewart J Levine
Oct 7, 2016·Frontiers in Pharmacology·Elizabeth M GordonStewart J Levine