High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro

Journal of Immunology Research
Johanna L GrünGalileo Escobedo

Abstract

The effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into metabolic syndrome patients and controls. Monocyte subpopulations were quantified by flow cytometry, and interleukin- (IL-) 1β secretion levels were measured by ELISA. Primary human monocytes were cultured in low or elevated concentrations of high-density lipoprotein (HDL) and stimulated with lipopolysaccharide (LPS). The nonclassical monocyte (NCM) percentage was significantly increased in metabolic syndrome patients as compared to controls, whereas classical monocytes (CM) were reduced. Among all metabolic syndrome risk factors, HDL reduction exhibited the most important correlation with monocyte subpopulations and then was studied in vitro. Low HDL concentration reduced the CM percentage, whereas it increased the NCM percentage and IL-1β secretion in LPS-treated monocytes. The LPS effect was abolished when monocytes were cultured in elevated HDL concentrations. Concurring with in vitro results, IL-1β serum values significantly incre...Continue Reading

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Citations

Jul 22, 2019·International Journal of Molecular Sciences·Kamil Jonas, Grzegorz Kopeć
Nov 28, 2019·Journal of Clinical Medicine·Gemma Chiva-Blanch, Lina Badimon
Jun 12, 2019·Journal of Immunology Research·Angélica Y Gómez-ArauzGuillermo Meléndez

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
density gradient centrifugation
flow cytometry

Software Mentioned

BD FACSDiva
BD [UNK]
GraphPad Prism

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