High density peptide microarrays. In situ synthesis and applications

Molecular Diversity
Xiaolian GaoXiaochuan Zhou

Abstract

The technologies enabling the creation of large scale, miniaturized peptide or protein microarrays are emerging. The focuses of this review are the synthesis and applications of peptide and peptidomimetic microarrays, especially the light directed parallel synthesis of individually addressable high density peptide microarrays using a novel photogenerated reagent chemistry and digital photolithography (Gao et al., 1998, J. Am. Chem. Soc. 120, 12698; Pellois et al. 2002, Nat. Biotechnol. 20, 922). Concepts related to the synthesis are discussed, such as the reactions of photogenerated acids in the deprotection step of peptide synthesis or oligonucleotide synthesis, and the applications of high density peptide chips in antibody binding assays are discussed. Peptide chips provide versatile tools for probing antigen-antibody, protein-protein, peptide-ligand interactions and are basic components for miniaturization, automation, and system integration in research and clinical diagnosis applications.

Citations

Jan 19, 2011·Chemical Society Reviews·Chen KatzAssaf Friedler
May 15, 2013·Expert Opinion on Drug Delivery·Saradha ChandrasekharVasant V Ranade
Jun 15, 2016·PloS One·Kurt WhittemoreLuhui Shen
Sep 1, 2005·Applied Microbiology and Biotechnology·Dinesh Kumar, Tek Chand Bhalla
Dec 22, 2010·Biointerphases·M Uttamchandani, S Moochhala
May 6, 2017·Chemical Communications : Chem Comm·B RidderM A R Meier
Feb 15, 2007·Nature Reviews. Molecular Cell Biology·Christopher M Overall, Carl P Blobel
Sep 29, 2018·Toxins·Line LedsgaardAndreas Hougaard Laustsen
Nov 11, 2010·Molecular & Cellular Proteomics : MCP·Rebecca F HalperinStephen Albert Johnston

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.