High-Density SNP Map Construction and QTL Identification for the Apetalous Character in Brassica napus L

Frontiers in Plant Science
Xiaodong WangJiefu Zhang

Abstract

The apetalous genotype is a morphological ideotype for increasing seed yield and should be of considerable agricultural use; however, only a few studies have focused on the genetic control of this trait in Brassica napus. In the present study, a recombinant inbred line, the AH population, containing 189 individuals was derived from a cross between an apetalous line 'APL01' and a normally petalled variety 'Holly'. The Brassica 60 K Infinium BeadChip Array harboring 52,157 single nucleotide polymorphism (SNP) markers was used to genotype the AH individuals. A high-density genetic linkage map was constructed based on 2,755 bins involving 11,458 SNPs and 57 simple sequence repeats, and was used to identify loci associated with petalous degree (PDgr). The linkage map covered 2,027.53 cM, with an average marker interval of 0.72 cM. The AH map had good collinearity with the B. napus reference genome, indicating its high quality and accuracy. After phenotypic analyses across five different experiments, a total of 19 identified quantitative trait loci (QTLs) distributed across chromosomes A3, A5, A6, A9 and C8 were obtained, and these QTLs were further integrated into nine consensus QTLs by a meta-analysis. Interestingly, the major QTL ...Continue Reading

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Citations

May 8, 2018·Plant Biotechnology Journal·Iulian GaburChristian Obermeier
Jun 8, 2018·Journal of Integrative Plant Biology·Chengqi LiJiafeng Song
Nov 25, 2016·Frontiers in Plant Science·Cunmin QuJia-Na Li
Jun 17, 2018·G3 : Genes - Genomes - Genetics·Erin E HigginsIsobel A P Parkin
Dec 11, 2021·Frontiers in Plant Science·Xiaozhen ZhaoXiaodong Wang

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Methods Mentioned

BETA
PCR
Assay
genotyping

Software Mentioned

SEA
BioMercator
JoinMap
G3DH
Perl script
GenomeStudio
SPSS
Windows QTL Cartographer

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