High developmental potential in vitro and in vivo of cattle embryos cloned without micromanipulators.

Journal of Assisted Reproduction and Genetics
Lleretny RodríguezF O Castro

Abstract

In order to simplify cloning, a new method that does not require micromanipulators was used. We aimed to evaluate the developmental potential of two bovine cell lines upon cloning. In vitro matured bovine oocytes, were released from zona pellucida, enucleated, fused to foetal or adult somatic donor cells. The reconstructed embryos were reprogrammed, activated and cultured until blastocyst stage. No micromanipulators were used. Blastocyst rate and quality was scored. Some expanded (d7) blastocysts were transferred to recipient cattle and collected back at d17 to assess elongation. High developmental potential in vitro of cloned embryos to expanded (d7) blastocysts was achieved (52.6%). In one cell line, 65.7% of blastocysts was scored. Most blastocysts (87.4%) were graded as excellent. In vivo development to elongation (day-17) in temporary recipient cows also showed a high developmental potential (11/18 transferred blastocysts elongated). Hand-made cloning is an efficient alternative for cloning in cattle.

References

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Citations

Jul 13, 2013·In Vitro Cellular & Developmental Biology. Animal·Lleretny Rodríguez-AlvarezFidel Ovidio Castro
Jun 9, 2009·Zygote : the Biology of Gametes and Early Embryos·Lleretny Rodríguez-AlvarezFidel Ovidio Castro
May 1, 2010·Zygote : the Biology of Gametes and Early Embryos·Lleretny Rodríguez-AlvarezFidel Ovidio Castro
Dec 17, 2014·Zygote : the Biology of Gametes and Early Embryos·Alejandra E VelasquezLleretny Rodriguez-Alvarez
May 26, 2017·PloS One·Edwin A MellishoLleretny Rodríguez-Álvarez
Dec 15, 2018·Molecular Reproduction and Development·Alejandra VelásquezLleretny Rodríguez-Álvarez
Apr 18, 2021·Journal of Assisted Reproduction and Genetics·Gábor VajtaSergey Yakovenko

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