High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins.

Journal of Biomolecular NMR
Anna Zawadzka-KazimierczukLibor Krásný

Abstract

Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.

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Citations

Jun 12, 2012·Journal of Biomolecular NMR·Wolfgang BermelJan Stanek
Jul 19, 2012·Journal of Biomolecular NMR·Anna Zawadzka-KazimierczukMartin Billeter
Nov 28, 2012·Biomolecular NMR Assignments·Leonhard GeistRobert Konrat
Jan 23, 2013·Biomolecular NMR Assignments·Zsuzsanna Orbán-NémethRobert Konrat
Oct 20, 2014·Journal of Biomolecular NMR·Alessandro PiaiIsabella C Felli
Sep 11, 2014·Journal of Biomolecular NMR·Saurabh SaxenaWiktor Koźmiński
Sep 7, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simone KosolPeter Tompa
Jan 21, 2016·Biophysical Journal·Alessandro PiaiRoberta Pierattelli
Jan 11, 2016·Biomolecular NMR Assignments·Andrea Gabriele FlammNicolas Coudevylle
Aug 21, 2015·Magnetic Resonance in Chemistry : MRC·Krzysztof Kazimierczuk, Vladislav Orekhov
Jun 25, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Krzysztof KazimierczukWiktor Koźmiński
Jun 25, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jaka KrageljMalene Ringkjøbing Jensen
Oct 13, 2012·Chembiochem : a European Journal of Chemical Biology·Wolfgang BermelRoberta Pierattelli
Nov 26, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Michał NowakowskiWiktor Koźmiński
Jan 27, 2015·Biomolecular NMR Assignments·Gerald PlatzerRobert Konrat
Apr 12, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christoph WiedemannRamadurai Ramachandran
Apr 24, 2015·Journal of Biomolecular NMR·Paweł DziekańskiAnna Zawadzka-Kazimierczuk
Mar 25, 2014·Journal of Magnetic Resonance·Isabella C Felli, Roberta Pierattelli
Mar 25, 2014·Journal of Magnetic Resonance·Robert Konrat
Mar 25, 2014·Journal of Magnetic Resonance·Jiří NováčekVladimír Sklenář
Jul 9, 2016·Journal of the American Chemical Society·Asmit BhowmickTeresa Head-Gordon
Dec 6, 2016·Angewandte Chemie·Dennis KurzbachGeoffrey Bodenhausen
Jan 14, 2017·Journal of the American Chemical Society·Maria BaiasLucio Frydman
Mar 2, 2018·Journal of Biomolecular NMR·Maria Grazia MurraliIsabella C Felli
Oct 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·João M C TeixeiraMiquel Pons
Mar 10, 2017·Biomolecular NMR Assignments·Cesyen CedeñoWiktor Koźmiński

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Methods Mentioned

BETA
NMR

Software Mentioned

SMFT
SPARKY

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