DOI: 10.1101/490789Dec 13, 2018Paper

High-dimensional characterization of IL-10 production and IL-10 dependent regulation during primary gammaherpesvirus infection

BioRxiv : the Preprint Server for Biology
Abigail K. KimballEric T. Clambey

Abstract

Interleukin (IL)-10 is a potent immunomodulatory cytokine produced by multiple cell types to restrain immune activation. Many herpesviruses have utilized the IL-10 pathway to facilitate infection, but how endogenous IL-10 is regulated during primary infection in vivo remains poorly characterized. Here, we infected mice with murine gammaherpesvirus 68 (γHV68) and analyzed the production, and genetic contribution, of IL-10 using mass cytometry, or cytometry by time-of-flight (CyTOF), analysis. γHV68 infection elicited a breadth of effector CD4 T cells in the lungs of acutely infected mice, including a highly activated effector subset that co-expressed IFNγ, TNFα, and IL-10. By using IL-10 green fluorescent protein (gfp) transcriptional reporter mice, we identified that IL-10 was primarily expressed within CD4 T cells during acute infection in the lungs. IL10gfp expressing CD4 T cells were highly proliferative and characterized by the expression of multiple co-inhibitory receptors including PD-1 and LAG-3. When we analyzed acute γHV68 infection of IL-10 deficient mice, we found that IL-10 limits the frequency of both myeloid and effector CD4 T cell subsets in the infected lung, with minimal changes at a distant mucosal site. These...Continue Reading

Related Concepts

Herpesviridae
Lung
Laboratory mice
CD4 Positive T Lymphocytes
Transcription, Genetic
Cytokine
T-Lymphocyte Subsets
Interleukin-10
Green Fluorescent Proteins
Site

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

American Thoracic Society: Allergy, Immunology & Inflammation

This feed has been developed in conjunction with the American Thoracic Society for the benefit of its Allergy, Immunology, and Inflammation Assembly. It highlights new and impactful papers on allergy, asthma, genetics, and the pathogenesis of lung diseases.