High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes.

Nature Communications
Karl ZhanghaoDayong Jin

Abstract

Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles' biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

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Citations

Mar 7, 2021·Critical Reviews in Oncology/hematology·Wenhui ZhangXiao Xu
May 25, 2021·Frontiers in Molecular Biosciences·Massimiliano GalluzziJiangyu Li
Jun 6, 2021·Biochemical and Biophysical Research Communications·Nori NakaiSumio Terada
Sep 24, 2021·Light, Science & Applications·Chao HeMartin J Booth
Nov 28, 2021·Light, Science & Applications·Ying Zhang

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Methods Mentioned

BETA
fluorescence polarization microscopy

Software Mentioned

ThunderSTORM
Matlab
Micro
Github
SIM
Fiji
SPSS
GraphPad Prism
manager
WF

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