High Diversity of Anaerobic Alkane-Degrading Microbial Communities in Marine Seep Sediments Based on (1-methylalkyl)succinate Synthase Genes

Frontiers in Microbiology
Marion StagarsKatrin Knittel

Abstract

Alkanes comprise a substantial fraction of crude oil and are prevalent at marine seeps. These environments are typically anoxic and host diverse microbial communities that grow on alkanes. The most widely distributed mechanism of anaerobic alkane activation is the addition of alkanes to fumarate by (1-methylalkyl)succinate synthase (Mas). Here we studied the diversity of MasD, the catalytic subunit of the enzyme, in 12 marine sediments sampled at seven seeps. We aimed to identify cosmopolitan species as well as to identify factors structuring the alkane-degrading community. Using next generation sequencing we obtained a total of 420 MasD species-level operational taxonomic units (OTU0.96) at 96% amino acid identity. Diversity analysis shows a high richness and evenness of alkane-degrading bacteria. Sites with similar hydrocarbon composition harbored similar alkane-degrading communities based on MasD genes; the MasD community structure is clearly driven by the hydrocarbon source available at the various seeps. Two of the detected OTU0.96 were cosmopolitan and abundant while 75% were locally restricted, suggesting the presence of few abundant and globally distributed alkane degraders as well as specialized variants that have deve...Continue Reading

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Citations

Nov 1, 2016·Nature·Rafael Laso-PérezFlorin Musat
May 16, 2017·Frontiers in Microbiology·Marion H StagarsKatrin Knittel
Nov 8, 2017·Frontiers in Microbiology·Rajesh SinghArpita Bose
Dec 12, 2020·Frontiers in Microbiology·Krisztián LacziKatalin Perei
Aug 25, 2021·Chemosphere·Lucélia CabralLara Durães Sette

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Datasets Mentioned

BETA
EF077227
AB468588
LN610408
LN610424
HG764719
HG764728
LM2801

Methods Mentioned

BETA
geneFISH
PCRs
PCR
Fluorescence
FACS

Software Mentioned

PolyPro
mothur
PhyML
BLASTX
Arb Silva
RevTrans
R
GeneFISH
ggplot2
UCHIME

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