High Diversity of Myocyanophage in Various Aquatic Environments Revealed by High-Throughput Sequencing of Major Capsid Protein Gene With a New Set of Primers

Frontiers in Microbiology
Weiguo HouHailiang Dong

Abstract

Myocyanophages, a group of viruses infecting cyanobacteria, are abundant and play important roles in elemental cycling. Here we investigated the particle-associated viral communities retained on 0.2 μm filters and in sediment samples (representing ancient cyanophage communities) from four ocean and three lake locations, using high-throughput sequencing and a newly designed primer pair targeting a gene fragment (∼145-bp in length) encoding the cyanophage gp23 major capsid protein (MCP). Diverse viral communities were detected in all samples. The fragments of 142-, 145-, and 148-bp in length were most abundant in the amplicons, and most sequences (>92%) belonged to cyanophages. Additionally, different sequencing depths resulted in different diversity estimates of the viral community. Operational taxonomic units obtained from deep sequencing of the MCP gene covered the majority of those obtained from shallow sequencing, suggesting that deep sequencing exhibited a more complete picture of cyanophage community than shallow sequencing. Our results also revealed a wide geographic distribution of marine myocyanophages, i.e., higher dissimilarities of the myocyanophage communities corresponded with the larger distances between the sampl...Continue Reading

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Citations

Mar 21, 2021·Applied and Environmental Microbiology·Julia A Gustavsen, Curtis A Suttle

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Datasets Mentioned

BETA
MG267122

Methods Mentioned

BETA
PCR
electrophoresis
Illumina sequencing

Software Mentioned

FastTree
UCULST
BlastN
MUSCLE
Cutadapt
QIIME
BlastP
SPSS Statistics
CLC Sequence Viewer
CLUSTER

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