High-dose insulin inhibits gap junction intercellular communication in vascular smooth muscle cells

Molecular Medicine Reports
Ou BianDingyin Zeng

Abstract

Gap junction intercellular communication (GJIC) is important in mediating intercellular substance and signal transmission. Connexin (Cx)43 is a major component involved in GJIC in vascular tissue and its abnormal expression is closely associated with various vascular diseases. Insulin resistance is the central component of metabolic syndrome, and high doses of insulin can affect vascular function through multiple pathways, resulting in cardiovascular disease. However, the effects of insulin on GJIC function and connexin (Cx)43 expression in vascular smooth muscle cells (VSMCs) remain unclear. Following treatment of VSMCs with different doses of insulin, a fluorescence recovery after photobleaching (FRAP) assay was performed to evaluate GJIC function in treated VSMCs. The results showed that high‑dose insulin suppressed GJIC function. Western blot assays further demonstrated that high‑dose insulin induced the phosphorylation of Cx43 at s368 and downregulated the expression of Cx43. H2O2 release assays demonstrated that high‑dose insulin treatment significantly elevated the cellular H2O2 level. In addition, compared with cells treated with high‑dose insulin, pretreatment with catalase significantly restored the cellular GJIC func...Continue Reading

References

May 10, 2001·The Journal of Clinical Endocrinology and Metabolism·C WeyerP A Tataranni
Oct 31, 2001·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·T InoguchiH Nawata
Feb 9, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Brenda R KwakFrançois Mach
Jul 20, 2002·Molecular Membrane Biology·W Howard Evans, Patricia E M Martin
Jul 5, 2003·Cell Biology International·Petula CoutinhoDavid Becker
Sep 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Douglas B CowanFrancis X McGowan
Apr 20, 2004·Cardiovascular Research·Jacques-Antoine HaefligerPaolo Meda
Apr 20, 2004·Cardiovascular Research·David P SlovutR Clinton Webb
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Scott EarleyBenjimen R Walker
Sep 24, 2004·Physiology·Xavier F FigueroaBrian R Duling
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Ki Won LeeChang Yong Lee
Jul 14, 2005·Annual Review of Nutrition·Gerald M Reaven
Jan 6, 2006·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Tadashi FujiwaraKazuaki Shimamoto
Mar 3, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Yongbo LiaoBrian R Duling
May 26, 2007·Endocrine Reviews·Ranganath MuniyappaMichael J Quon
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Ranganath Muniyappa, Michael J Quon
Jul 3, 2008·Cardiovascular Research·Matheus L RochaLusiane M Bendhack
Oct 4, 2008·Antioxidants & Redox Signaling·Xavier F Figueroa, Brian R Duling
Aug 19, 2009·Archives of Physiology and Biochemistry·Giovanni AnfossiMariella Trovati
Oct 10, 2009·International Review of Cell and Molecular Biology·Scott JohnstoneDarren Locke
Jul 6, 2010·Journal of Cardiovascular Pharmacology and Therapeutics·Jing ShenShen-Jiang Hu
Sep 3, 2010·Current Vascular Pharmacology·Danna M Breen, Adria Giacca
Dec 17, 2010·Trends in Pharmacological Sciences·Tony Tiganis
Dec 18, 2010·Free Radical Biology & Medicine·Erik J HenriksenElizabeth M Marchionne
Jun 28, 2012·Frontiers in Physiology·Chintamani N JoshiDavid A Tulis
Jul 20, 2012·Journal of Agricultural and Food Chemistry·Wei-Yun ZhangChang-Seon Myung
Jan 11, 2013·American Journal of Physiology. Cell Physiology·Rajkumar PylaLakshman Segar
May 15, 2013·Archives of Medical Science : AMS·Jian Ming WeiYunzeng Zou

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Citations

Oct 3, 2018·Current Vascular Pharmacology·Tamara SáezLuis Sobrevia

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