High-efficiency biosynthesis of hypocrellin A in Shiraia sp. using gamma-ray mutagenesis

Applied Microbiology and Biotechnology
Xin-Yao LiuCheng-Lin Hou

Abstract

Hypocrellin A (HA), well known as one of the best natural pigments and bioactive agent to treat skin diseases, is further anticipated to play a vital role in photodynamic therapy (PDT) in anticancer and antiviral treatments. In this study, an HA-producing strain ZZZ816 (Shiraia sp.) was isolated from the moso bamboo (Phyllostachys edulis) seeds, and gamma irradiation was used to mutagenize spores of the original strain. After treatment with cobalt-60 gamma ((60)Coγ) with different doses (20, 50, 80, 100, 150, 180, 300, and 500 Gy), the 100 Gy was selected as the optimal condition, which led to 77.2 % lethality of spores and 35 % positive mutant frequency. The extracted compound of the most excellent HA-producing strain (H-4-2) was precisely analyzed by a combination of seven detection methods, and the maximum HA content was shown to reach 2018.3 mg/L. HA production in H-4-2 increased by 414.9 % compared to that of original strain ZZZ816 (392 mg/L) and was significantly higher than all the other industrial HA-producing strains in published reports.

References

Aug 1, 1991·Planta medica·T KishiS Takahashi
Nov 1, 1972·Antimicrobial Agents and Chemotherapy·J W Brandsberg, M E French
Jun 1, 1995·Photochemistry and Photobiology·Z Diwu
Sep 1, 1994·Photochemistry and Photobiology·J B HudsonG H Towers
Sep 25, 1999·Journal of Biomolecular Structure & Dynamics·E KocisováJ V García-Ramos
Dec 19, 2001·Journal of Photochemistry and Photobiology. B, Biology·S M AliM Olivo
Nov 1, 2002·Biochemical and Biophysical Research Communications·Martin H DeiningerHermann J Schluesener
Aug 23, 2011·Canadian Journal of Microbiology·Naimeh NajafiShokoofeh Golkhoo
Dec 12, 2012·Folia Microbiologica·Wen DuChunlong Sun

❮ Previous
Next ❯

Citations

Oct 20, 2018·Photochemistry and Photobiology·Yan Jun MaJian Wen Wang
Sep 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng QinYang Zeng
Feb 2, 2019·The Journal of Microbiology·Dan LiShu-Zhen Yan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.