High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation

Stem Cell Research & Therapy
Chuti LaowtammathronSurapol Issaragrisil

Abstract

Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The first hESC line was generated in 1998 by culturing inner cell mass (ICM) cells isolated from human blastocysts using an immunosurgery technique. Since then, many techniques including mechanical ICM isolation, laser dissection, and whole embryo culture have been used to derive hESC lines. However, the hESC derivation efficiency remains low, usually less than 50%, and it requires a large number of human embryos to derive a significant number of hESC lines. Due to a shortage of and restricted access to human embryos, a novel approach with better hESC derivation efficiency is badly needed to decrease the number of embryos used. We hypothesized that the low hESC derivation efficiency might be due to extensive proliferation of trophoblast (TE) cells which could interfere with ICM proliferation. We therefore developed a methodology to minimize TE cell proliferation by culturing ICM in a feeder-free system for 3 days before transferring them onto feeder cells. This minimized trophoblast cell proliferation ...Continue Reading

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Mar 1, 1989·Fertility and Sterility·L J WiltonA O Trounson
Jan 1, 1988·Journal of Reproduction and Fertility·M Monk, A H Handyside
Nov 6, 1998·Science·J A ThomsonJ M Jones
Mar 5, 2004·The New England Journal of Medicine·Chad A CowanDouglas A Melton
May 12, 2005·Lancet·Irina KlimanskayaRobert Lanza
May 3, 2008·Stem Cells·Neta LavonNissim Benvenisty
Oct 22, 2008·Cell Stem Cell·Jeremy Micah CrookAlan Colman
Apr 28, 2009·Nature Medicine·Evelyne VannesteJoris R Vermeesch
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bao-Yang HuSu-Chun Zhang
Mar 4, 2010·In Vitro Cellular & Developmental Biology. Animal·Susanne StrömOuti Hovatta
Jul 20, 2010·Stem Cells·Juan-Carlos BiancottiNeta Lavon
Dec 9, 2010·Development·Jennifer Nichols, Austin Smith
Nov 15, 2011·Human Reproduction·Kirstine KirkegaardHans Jakob Ingerslev
Jun 23, 2012·Seminars in Reproductive Medicine·Kangpu Xu, Markus Montag
Jul 17, 2014·Human Reproduction·S SampinoG E Ptak
Jul 4, 2015·Annals of Medicine·Oscar E SimonsonSergey Rodin
Mar 19, 2016·Stem Cells Translational Medicine·Moustafa M El KhatibYasuhiro Ikeda
Oct 4, 2016·Journal of Assisted Reproduction and Genetics·Liron Bar-ElDalit Ben-Yosef
Oct 11, 2016·Reproductive Biomedicine Online·Krithika RavichandranSantiago Munné

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Methods Mentioned

BETA
dissection
biopsy
reverse transcription PCR
PCR
electrophoresis

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