High efficiency hydrogen production from glucose/xylose by the ldh-deleted Thermoanaerobacterium strain

Bioresource Technology
Shuang LiXiaoning Wang

Abstract

A strictly anaerobic, thermoacidophilic, H(2)-producing bacterium was isolated and designated as Thermoanaerobacterium aotearoense. The optimized cultivation conditions for H(2) production are 55 degrees C, pH 6.5 and 10gl(-1) of glucose or xylose. A metabolic pathway analysis showed that lactate occupied most of the liquid metabolites and consumed a large amount of NADH. To increase the efficiency of hydrogen production, the gene encoding the l-lactate dehydrogenase was knocked out to redirect the NADH flow. Genetic manipulation resulted in the 2 and 2.5 folds increase of the H(2) yield and production rate, respectively. The maximum H(2) yields using the Deltaldh mutant were 2.71, 1.45 and 2.28molH(2)mol(-1) sugar under glucose, xylose and glucose/xylose mixture tests, respectively. The recombinant Deltaldh strain could ferment the mixture of glucose and xylose to produce H(2) effectively, indicating that the performance of Thermoanaerobacterium in H(2) production can be significantly improved by metabolic engineering technique.

References

Feb 9, 2002·Applied Microbiology and Biotechnology·H H P FangH Liu
Apr 9, 1965·Science·C T GRAY, H GEST
Mar 30, 2004·Biochemical Society Transactions·P SommerB K Ahring
May 10, 2006·Applied Microbiology and Biotechnology·Akihito YoshidaHideaki Yukawa
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 17, 2007·Applied Microbiology and Biotechnology·Toshinari MaedaThomas K Wood
May 27, 2008·Biotechnology and Bioengineering·Perttu E P KoskinenJaakko A Puhakka
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·A Joe ShawLee R Lynd
Mar 31, 2009·Trends in Biotechnology·Patrick C Hallenbeck, Dipankar Ghosh
Feb 13, 2010·Bioresource Technology·Maria Assunta BasileGiovanna Gomez d'Ayala
Feb 16, 2010·Extremophiles : Life Under Extreme Conditions·Ida RomanoAgata Gambacorta

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Citations

Mar 7, 2013·Canadian Journal of Microbiology·Yogesh GoyalKalyan Gayen
Apr 8, 2011·Bioresource Technology·Mona Abo-HasheshPatrick C Hallenbeck
Mar 15, 2011·Bioresource Technology·R Y LiH H P Fang
Aug 1, 2013·Bioresource Technology·Jingrong Cheng, Mingjun Zhu
Jan 1, 2012·Computational and Structural Biotechnology Journal·Roberto Mazzoli
Feb 7, 2012·Biotechnology Advances·Feng PengRun-Cang Sun
Sep 27, 2019·Biotechnology Journal·Shaojie WangPing Xu
Sep 1, 2017·Scientific Reports·Muzi ZhuXiaoning Wang

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