High efficiency introduction of plasmid DNA into glycine treated Enterococcus faecalis by electroporation

Molecular & General Genetics : MGG
A L Cruz-Rodz, M S Gilmore

Abstract

A highly efficient electroporation system for Enterococcus faecalis was developed by systematically optimizing different parameters. One parameter found to be particularly critical for electroporation was cultivation of E. faecalis in medium containing a high glycine concentration, prior to electroporation. Osmotic stabilization of cells with 0.5 M sucrose was also found to be critical during glycine treatment. 10(6) transformants per microgram of plasmid DNA were consistently obtained within 48 h. Electrocompetent preparations of E.-faecalis could be stored at - 70 degrees C without loss of competence.

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Citations

Dec 1, 2009·Applied Microbiology and Biotechnology·Trond Erik Vee Aune, Finn Lillelund Aachmann
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lynn E Hancock, Michael S Gilmore
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Sep 9, 2008·Journal of Bacteriology·María Florencia Del Papa, Marta Perego
Oct 9, 2007·Journal of Bacteriology·Maria Florencia Del PapaMarta Perego
Jan 25, 2011·Journal of Bacteriology·María Florencia Del Papa, Marta Perego
Nov 15, 2011·Journal of Bacteriology·Vijayalakshmi S Iyer, Lynn E Hancock
Jul 18, 2003·Journal of Bacteriology·Lynn E HancockMichael S Gilmore
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Nov 24, 2004·Molecular Microbiology·Marie-Cécile LamyClaire Poyart

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