High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments

Journal of Neuroscience Methods
Carsten HagemannGiles Hamilton Vince

Abstract

In order to investigate the impact of signalling proteins on the phenotype and malignant behavior of glioblastoma cells, we optimized the transfection procedure of human glioblastoma cell lines U251, U373, GaMG and of primary cells obtained from a patient's tumor using nucleofection technology in conjunction with plasmid pmaxGFP. We describe the optimization procedure, show that a high percentage of the cells can be transfected and that nucleofection does not cause phenotypic alterations of the cells. Therefore, we conclude that nucleofection is a highly efficient tool to deliver plasmids for transient protein overexpression and siRNA for specific protein knock-down to different glioblastoma cell lines or primary cells.

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Citations

Mar 20, 2014·BMC Research Notes·Almuth Friederike KesslerCarsten Hagemann
Aug 24, 2010·Neurochemical Research·H MuydermanN R Sims
Jul 22, 2008·Nanomedicine : Nanotechnology, Biology, and Medicine·Daniela Reischl, Andreas Zimmer
Mar 4, 2008·Experimental Dermatology·Sandra Y HanDavid Polsky
Sep 22, 2010·Journal of Cellular Physiology·Antony HsiehDavid Hsieh
Aug 1, 2015·PLoS Neglected Tropical Diseases·Kimbra G TurnerMiguel A Morales

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