High endogenous nitrogenase activity in isolated heterocysts of Anabaena sp. strain CA after nitrogen starvation.

Journal of Bacteriology
A KumarC Van Baalen

Abstract

Metabolically active heterocysts were isolated from a mutant of Anabaena sp. strain CA with fragile vegetative cells. Heterocysts isolated from cultures grown in 1% CO2 in air reduced C2H2 at 57 and 10 nmol of C2H2 per mg (dry weight) per min under H2 and Ar, respectively. However, if whole filaments were sparged with 1% CO2 in 99% Ar for 12 h before heterocyst isolation, these heterocysts showed C2H2 reduction rates of 83 nmol of C2H4 per mg (dry weight) per min under either H2 or Ar, or 40% the activity of whole filaments grown in 1% CO2 in air. Heterocysts isolated from cultures sparged with 100% Ar or 1% CO2 in 99% N2 had the same C2H2 reduction pattern as heterocysts from cultures grown in 1% CO2 in air, i.e., low activity under Ar and high activity under H2. Labeling of whole filaments incubated with NaH14CO3 for 12 h under 1% CO2 in air or 1% CO2 in 99% Ar resulted in a twofold higher accumulation of 14C-labeled compounds in vegetative cells and heterocysts of Ar-incubated cells. Our results suggest that during incubation under 1% CO2 in 99% Ar, presumably a nitrogen starvation condition, continuing photosynthetic fixation of CO2 leads to accumulation of material(s) in the heterocysts that supports a high, persistent end...Continue Reading

References

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Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R B Peterson, C P Wolk

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Citations

Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
May 1, 1985·Journal of Bacteriology·R L SmithC Van Baalen
Jun 1, 1992·Microbiological Reviews·P Fay
Nov 13, 2004·Journal of Plant Physiology·Radha PrasannaPawan Kumar Singh

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