High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.

BMC Immunology
Yilmaz MahshidHans-Erik Claesson

Abstract

Human B lymphocytes can produce leukotriene B4 but the biological function of the 5-lipoxygenase (5-LO) pathway in B cells is unclear. In order to better understand and define the role of 5-LO in B cells, we investigated the expression of 5-LO mRNA and protein in subsets of B cells from human tonsils and different types of B cell lymphoma. Based on RT-PCR and western blot/immunohistochemical staining, with a polyclonal antibody raised against 5-LO, high expression of 5-LO was found in mantle zone B cells from tonsils. By contrast, only a weak expression of 5-LO was detected in germinal centre cells and no expression in plasma cells from tonsils. This pattern of 5-LO expression was preserved in malignant lymphoma with high expression in mantle B cell lymphoma (MCL) and weak or no expression in follicular lymphoma. Primary leukemized MCL, so called B-prolymphocytic leukaemia cells, and MCL cell lines also expressed 5-LO and readily produced LTB4 after activation. The present report demonstrates the expression of 5-LO mainly in normal and malignant mantle zone B cells while the expression is low or absent in germinal centre B cells and plasma cells, indicating a role of the 5-LO pathway in B cells before the cells finally differen...Continue Reading

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Citations

Apr 7, 2009·Molecular & Cellular Proteomics : MCP·Robert S BoydKelvin Cain
Jul 4, 2013·The Journal of Pharmacology and Experimental Therapeutics·Richard J JonesRobert Z Orlowski
Feb 26, 2010·Expert Opinion on Therapeutic Patents·Carlo Pergola, Oliver Werz
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Jun 12, 2021·Prostaglandins & Other Lipid Mediators·Chuanyou XiaHans-Erik Claesson

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Methods Mentioned

BETA
flow cytometry
biopsies
biopsy
FCS
PCR

Software Mentioned

LSM510 Image Examiner
Picsara

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