High Expression of ABL2 Suppresses Apoptosis in Gastric Cancer

Digestive Diseases and Sciences
Yun LiuShihe Shao

Abstract

Diseases associated with Abelson-related gene (also called ABL2) include leukemia; furthermore, previous researches have studied the expressions and functions of ABL2 in different types of malignancies and found that it plays an important role in almost all kinds of cancers. Nevertheless, the mechanism of ABL2 in gastric cancer (GC) remains vague. In the present study, the level of ABL2 in human GC tissues was detected by immunohistochemistry. Also, the GC cell lines MGC-803 and BGC-823 were selected to stably knock down and overexpress the level of ABL2 by corresponding lentiviral vectors. Puromycin was used to maintain the stable low expression of ABL2 MGC-803 cells compared with control cells; what is more, the high expression of ABL2 BGC-823 cells was also obtained. Based on it, we detected the proteins associated with apoptosis, such as Bcl-2 family and caspase family by western blotting. The most appropriate concentration of puromycin to kill GC cells is 1 µg/mL; then, we obtained the corresponding stable cell lines. Furthermore, we found that high level of ABL2 in BGC-823 cells increased the expression of Bcl-XL, total PARP, and caspase3, while decreased the level of cleaved caspase3 and cleaved caspase9. Consistent resu...Continue Reading

References

Aug 7, 2013·The International Journal of Biochemistry & Cell Biology·Meghan E Kerrisk, Anthony J Koleske
Dec 9, 2015·Trends in Cancer·Jun Wang, Ann Marie Pendergast
May 18, 2017·Nature Reviews. Gastroenterology & Hepatology·Nisha PadmanabhanPatrick Tan
Sep 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Raman DhivyaJamespandi Annaraj
Oct 7, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chae Hwa KwonDo Youn Park

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Citations

Nov 26, 2020·International Journal of Molecular Sciences·Justin F CreedenRobert E McCullumsmith
Feb 11, 2021·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Yun LiuShihe Shao

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