High expression of Copine 1 promotes cell growth and metastasis in human lung adenocarcinoma

International Journal of Oncology
Shunlin LiuJianan Huang

Abstract

Despite advances in diagnosis and treatment, the survival of non-small cell lung cancer (NSCLC) patients is poor. Further understanding of the disease mechanism and treatment strategies is required. Copines are a family of calcium-dependent phospholipid-binding proteins that are evolutionally conserved in various eukaryotic organisms and protists. Copine 1, encoded by CPNE1, is a soluble membrane-binding protein, which includes two tandem C2 domains at the N-terminus and an A domain at the C‑terminus. A previous study reported that Copine 1 binds with various intracellular proteins via its A domain and C  omain. However, the role of CPNE1 in lung cancer remains unclear. In the presented study, CPNE1 expression level was demonstrated to be positively associated with the stage (P=0.002) and significantly associated with lymph node status (P=0.011) and distant metastasis (P=0.042). Furthermore, the function of CPNE1 in regulation of cell growth, migration and invasion was investigated, and it was demonstrated that knockdown of CPNE1 inhibits the cell cycle in NSCLC cells. Collectively, these data suggest that CPNE1 is an oncogene in NSCLC and serves an important role in tumorigenesis of NSCLC progression.

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Citations

Nov 19, 2020·International Journal of Molecular Sciences·Cecilia Zavala-TecuapetlaHiram Luna-Munguia
Aug 10, 2021·Frontiers in Genetics·Haicheng TangFeng Li
Aug 17, 2021·Oxidative Medicine and Cellular Longevity·Zhen-Dong ZhangJian-Yong Li

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Methods Mentioned

BETA
transfection
fluorescence
fluorescence microscopy
light microscopy
xenograft

Software Mentioned

GraphPad Prism
SPSS
GraphPad

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