High expression of HIF-2α and its anti-radiotherapy effect in lung cancer stem cells

Genetics and Molecular Research : GMR
J C SunD L Niu

Abstract

Hypoxia-inducible factor-2 alpha (HIF-2α) has been shown to regulate cell stemness, although the expression and effects of HIF-2α in lung cancer stem cells remained unclear. This study investigated HIF-2α expression in lung cancer stem cells, as well as the relationship between HIF-2α expression and radioresistance in lung cancer cells. Stem-like cells (CD133(+)) in the non-small-cell lung cancer cell line A549 were enriched by serum-free culture conditions, and CD133(+) cells were sorted via fluorescence-activated cell sorting. A549 cells were treated with middle-infrared radiation, and the level of HIF-2α expression was determined by a quantitative polymerase chain reaction assay and western blot analysis. The level of HIF-2α expression in tissue sections from 50 cases of clinically confirmed non-small-cell lung cancer was determined via immunohistochemical analysis, and its correlation with prognosis after radiotherapy was analyzed. HIF-2α levels in CD133(+) cells were significantly higher than those in CD133(-) cells (P = 0.032). However, after radiation treatment, these levels were significantly upregulated in both CD133(+) and CD133(-) cells (P = 0.031 and P = 0.023, respectively). After irradiation, the proportions of ap...Continue Reading

Citations

Apr 11, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Ding ZhaoDongfeng Han
Aug 5, 2016·Oncotarget·Engeng ChenZhangfa Song
May 31, 2018·International Journal of Oncology·Wei-Wei TongYong Liu
Oct 17, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yifan WangSteven H Lin
May 1, 2021·Journal of Clinical Medicine·Elisa Stellaria GrassiLuca Persani

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