High Expression of HLA-G in Ovarian Carcinomatosis: The Role of Interleukin-1β

Neoplasia : an International Journal for Oncology Research
Matti UllahMassoud Mirshahi

Abstract

The present study focuses on the influence of the tumor microenvironment on the expression of HLA-G in ovarian cancer and its impact on immune cells. We used carcinomatosis fluids (n = 16) collected from patients diagnosed with epithelial ovarian cancer, detected by an increase in CA125 levels. Our results indicate that HLA-G is expressed by 1) ascitic cell clusters, 2) stromal cells (hospicells) extracted from cancer cell clusters, and 3) cancer cell lines and tumor cells. The origin of HLA-G was linked to inflammatory cytokines present in the cancer microenvironment. In parallel, the ascitic fluid of patients with ovarian cancer contains soluble HLA-G (sHLA-G). The mesothelial cell layer and submesothelial tissues, as well as the immune cell infiltrate, do not secrete HLA-G. In contrast, sHLA-G is absorbed by peritoneal tissues along with mesothelial layers as well as immune cell infiltrates. We demonstrated that interleukin-1β along with TGF-β can be a major HLA-G-inducing factor that upregulates HLA-G expression through the NF-κB pathway. The level of HLA-G in ascites correlated positively with the expression of T regulatory (T-regs) cells, while it negatively correlated with the expression of natural killer and memory cell...Continue Reading

Citations

Jan 18, 2020·Journal of Clinical Medicine·Cinzia FiondaAngela Santoni
Oct 2, 2020·International Journal of Immunogenetics·Antonio Arnaiz-VillenaJose M Martin-Villa
Nov 6, 2020·Neoplasia : an International Journal for Oncology Research·Iman Al DybiatMassoud Mirshahi
Nov 18, 2019·Neoplasia : an International Journal for Oncology Research·Shah ShahidMirshahi Massoud
Oct 26, 2020·Human Reproduction Update·M BourdonC Chapron
Mar 19, 2021·Klinicheskaia laboratornaia diagnostika·O V KovalevaN E Kushlinskii
Jul 13, 2021·Frontiers in Immunology·Xin LuoPing Yi
Jul 18, 2021·HLA : Immune Response Genetics·Wafa BabayInès Zidi
Aug 28, 2021·Frontiers in Immunology·Mikel Etxebeste-MitxeltorenaBeatriz Martín-Antonio

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Methods Mentioned

BETA
ELISAs
PCR
electrophoresis
flow cytometry
X-ray
ELISA
FACS
protein array

Software Mentioned

Multi Gauge
GraphPad Prism
GraphPad

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