High expression of hnRNPA1 promotes cell invasion by inducing EMT in gastric cancer

Oncology Reports
Yahua ChenZheng Guo

Abstract

Advanced gastric cancer (GC) has a poor prognosis and its treatment strategies are not very efficient. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) has emerged as a plausible GC marker, however the role and molecular mechanism of hnRNPA1 in cell invasion and migration remains unknown. In the present study, the gene expression across normal and tumor tissue (GENT) database was used to evaluate the mRNA expression of hnRNPA1 in various types of cancer. Western blot analysis (WB) and immunohistochemistry (IHC) were performed to detect the protein expression of hnRNPA1 in GC tissues and adjacent non‑tumor tissues. The expression of multiple oncogenes was detected by western blot analysis and quantitative RT‑PCR in hnRNPA1 overexpressing GC cells. Soft agar colony formation, EdU incorporation, wound healing and invasion assays were applied to verify the role of hnRNPA1 in anchorage‑independent cell growth, migration and invasion in GC cells. Epithelial‑to‑mesenchymal transition (EMT) markers were detected by immunofluorescence, western blot analysis and IHC in vitro. A nude mice model of metastasis carcinoma was established to confirm the role of hnRNPA1 during EMT in vivo. Our results revealed that hnRNPA1 was significantly...Continue Reading

Citations

Feb 13, 2019·International Journal of Molecular Sciences·Sergey S ShishkinKsenia Lisitskaya
Jun 11, 2020·International Journal of Molecular Sciences·Sathyen A PrabhuSonia V Del Rincón
Feb 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lavinia A CarabetArtem Cherkasov
Dec 4, 2021·Frontiers in Genetics·Somayah AlbaradeiXin Gao

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenograft

Software Mentioned

SPSS
ImageJ
Photoshop

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Ruixia XieDekui Zhang
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Hao WangShaofen Huo
© 2022 Meta ULC. All rights reserved