High expression of sphingosine kinase 1 and S1P receptors in chemotherapy-resistant prostate cancer PC3 cells and their camptothecin-induced up-regulation

Biochemical and Biophysical Research Communications
Yukihiro AkaoYoshinori Nozawa

Abstract

Although most of pharmacological therapies for cancer utilize the apoptotic machinery of the cells, the available anti-cancer drugs are limited due to the ability of prostate cancer cells to escape from the anti-cancer drug-induced apoptosis. A human prostate cancer cell line PC3 is resistant to camptothecin (CPT). To elucidate the mechanism of this resistance, we have examined the involvement of sphingosine kinase (SPHK) and sphingosine 1-phosphate (S1P) receptor in CPT-resistant PC3 and -sensitive LNCaP cells. PC3 cells exhibited higher activity accompanied with higher expression levels of protein and mRNA of SPHK1, and also elevated expression of S1P receptors, S1P(1) and S1P(3), as compared with those of LNCaP cells. The knockdown of SPHK1 by small interfering RNA and inhibition of S1P receptor signaling by pertussis toxin in PC3 cells induced significant inhibition of cell growth, suggesting implication of SPHK1 and S1P receptors in cell proliferation in PC3 cells. Furthermore, the treatment of PC3 cells with CPT was found to induce up-regulation of the SPHK1/S1P signaling by induction of both SPHK1 enzyme and S1P(1)/S1P(3) receptors. These findings strongly suggest that high expression and up-regulation of SPHK1 and S1P r...Continue Reading

References

Jul 17, 1999·British Journal of Cancer·A P Costa-Pereira, T G Cotter
Jul 6, 2000·FEBS Letters·S Spiegel, S Milstien
Aug 5, 2000·Biochemical Society Transactions·D K Perry
Dec 15, 2000·Current Biology : CB·P XiaM A Vadas
Mar 10, 2001·Journal of Neurochemistry·L C EdsallS Milstien
Jun 8, 2002·Biochemical and Biophysical Research Communications·Yukihiro AkaoYoshinori Nozawa
Jan 18, 2003·Biochimica Et Biophysica Acta·Michael MaceykaSarah Spiegel
May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
Feb 28, 2004·The Journal of Biological Chemistry·Tarek A TahaLina M Obeid
Aug 3, 2004·Nature Reviews. Cancer·Besim Ogretmen, Yusuf A Hannun
Feb 16, 2005·The Journal of Biological Chemistry·Tarek A TahaLina M Obeid
May 5, 2005·Molecular and Cellular Biology·Sravan K GoparajuSarah Spiegel
Nov 30, 2005·Molecular and Cellular Biology·Kiyomi MizugishiRichard L Proia
Dec 17, 2005·Cancer Research·Dimitri PchejetskiOlivier Cuvillier

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Citations

Oct 19, 2011·Cancer Metastasis Reviews·Hideki FuruyaToshihiko Kawamori
Jun 18, 2010·Nature Reviews. Cancer·Nigel J Pyne, Susan Pyne
Jul 19, 2011·Oncogene·L A Heffernan-StroudL M Obeid
Feb 8, 2013·Chemical Communications : Chem Comm·Dong Jae BaekRobert Bittman
Jun 3, 2011·The Journal of Pharmacology and Experimental Therapeutics·Perry C KennedyKevin R Lynch
Jul 3, 2010·Journal of Biomaterials Science. Polymer Edition·Xiaoying WangXinqiao Jia
May 4, 2011·World Journal of Biological Chemistry·Yuan-Li HuangHsinyu Lee
Oct 5, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao-Dong MengJun Xiao
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Babak OskouianJulie D Saba
Sep 15, 2006·FEBS Letters·Besim Ogretmen
Jun 24, 2008·Endothelium : Journal of Endothelial Cell Research·Vidya Limaye
Jan 8, 2013·Expert Opinion on Drug Metabolism & Toxicology·Young Mi KimSang Geon Kim
May 23, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·James R Van Brocklyn, Joseph B Williams
Oct 26, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Bernard MalavaudOlivier Cuvillier
Feb 20, 2010·Biochimie·Weng In Leong, Julie D Saba
Mar 3, 2010·Progress in Lipid Research·Patricia GangoitiAntonio Gómez-Muñoz
Jun 19, 2009·Advances in Enzyme Regulation·Nigel J PyneSusan Pyne
Feb 24, 2007·Journal of Neurochemistry·Anthony PilorgetRichard Béliveau
Nov 22, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Meiyan BaoXianghuo He
Jun 25, 2008·Biochemical and Biophysical Research Communications·Keitaro KojimaMasafumi Ito
Jan 21, 2012·Biochemical Society Transactions·Nigel J PyneSusan Pyne
Apr 15, 2011·Journal of Lipids·Wei-Ching HuangChiou-Feng Lin
Jul 16, 2008·Expert Opinion on Therapeutic Targets·Olivier Cuvillier
Mar 18, 2015·Biochemical and Biophysical Research Communications·Wenhua LiZhihua Ruan
Aug 14, 2013·Biochimica Et Biophysica Acta·Samy A F MoradMyles C Cabot
Nov 19, 2013·Biochimica Et Biophysica Acta·Marco Antonio Morquecho-LeónJ Adolfo García-Sáinz
Aug 3, 2016·Medicinal Research Reviews·Dominik Vogt, Holger Stark
Sep 5, 2017·International Journal of Molecular Sciences·Nahal HaddadiEileen M McGowan
Jan 13, 2018·Journal of Lipid Research·Michael J Pulkoski-GrossLina M Obeid

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis